Burke R A, Hughes G, Moberly J B
Renal Division, Baxter Healthcare Corporation, McGaw Park, Illinois, USA.
Adv Perit Dial. 1999;15:234-7.
Glucose has been reported to interfere in the analysis of creatinine by the Jaffe method. The potential interference of icodextrin and its primary metabolites (maltose, maltotriose, maltotetraose) on creatinine measurements has not previously been addressed. We evaluated the potential interference of icodextrin and its metabolites at various concentrations using both the Jaffe and Creatinine Plus methods. Interference was determined in samples containing 0.6-20 mg/dL creatinine in saline solution or in plasma (n = 6), and in dialysate samples (n = 6) spiked with icodextrin, maltose, maltotriose, and maltotetraose at concentrations up to twofold the level found in plasma and dialysate from patients treated using icodextrin. Results confirm that no interference occurs when using either the colorimetric Jaffe method or the enzymatic Creatinine Plus method at levels up to 65 g/L icodextrin, 2 g/L maltose, 2 g/L maltotriose, and 1 g/L maltotetraose, levels representing worst-case clinical concentrations. In addition, our results confirm that comparable values can be obtained using either the Jaffe or the Creatinine Plus method for the analysis of creatinine in uremic plasma and in dialysate samples.
据报道，葡萄糖会干扰用杰氏法分析肌酐。此前尚未探讨过艾考糊精及其主要代谢产物（麦芽糖、麦芽三糖、麦芽四糖）对肌酐测量的潜在干扰。我们使用杰氏法和肌酐加酶法评估了不同浓度的艾考糊精及其代谢产物的潜在干扰。在含有0.6 - 20mg/dL肌酐的生理盐水溶液或血浆样本（n = 6）以及添加了浓度高达使用艾考糊精治疗的患者血浆和透析液中发现的水平两倍的艾考糊精、麦芽糖、麦芽三糖和麦芽四糖的透析液样本（n = 6）中测定干扰情况。结果证实，当使用比色法杰氏法或酶法肌酐加酶法时，在艾考糊精浓度高达65g/L、麦芽糖浓度高达2g/L、麦芽三糖浓度高达2g/L和麦芽四糖浓度高达1g/L（代表最坏情况的临床浓度）时均不会产生干扰。此外，我们的结果证实，使用杰氏法或肌酐加酶法分析尿毒症血浆和透析液样本中的肌酐可获得相当的值。
