通道形成剂泽尔韦米星IIB在各向同性溶剂中的核磁共振结构。

NMR structure of the channel-former zervamicin IIB in isotropic solvents.

作者信息

Balashova T A, Shenkarev Z O, Tagaev A A, Ovchinnikova T V, Raap J, Arseniev A S

机构信息

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya, Moscow, Russia.

出版信息

FEBS Lett. 2000 Jan 28;466(2-3):333-6. doi: 10.1016/s0014-5793(99)01707-x.

DOI:10.1016/s0014-5793(99)01707-x

PMID:10682854

Abstract

Spatial structure of the membrane channel-forming hexadecapeptide, zervamicin IIB, was studied by NMR spectroscopy in mixed solvents of different polarity ranging from CDCl3/CD3OH (9:1, v/v) to CD3OH/H2O (1:1, v/v). The results show that in all solvents used the peptide has a very similar structure that is a bent amphiphilic helix with a mean backbone root mean square deviation (rmsd) value of ca. 0.3 A. Side chains of Trp1, Ile2, Gln3, Ile5 and Thr6 are mobile. The results are discussed in relation to the validity of the obtained structure to serve as a building block of zervamicin IIB ion channels.

摘要

采用核磁共振光谱法，在从CDCl3/CD3OH（9:1，v/v）到CD3OH/H2O（1:1，v/v）不同极性的混合溶剂中，研究了膜通道形成十六肽zervamicin IIB的空间结构。结果表明，在所有使用的溶剂中，该肽具有非常相似的结构，即弯曲的两亲性螺旋结构，平均主链均方根偏差（rmsd）值约为0.3 Å。色氨酸1、异亮氨酸2、谷氨酰胺3、异亮氨酸5和苏氨酸6的侧链是可移动的。结合所获得的结构作为zervamicin IIB离子通道构建单元的有效性对结果进行了讨论。