Georgieva Marina L, Bilanenko Elena N, Ponizovskaya Valeria B, Kokaeva Lyudmila Y, Georgiev Anton A, Efimenko Tatiana A, Markelova Natalia N, Kuvarina Anastasia E, Sadykova Vera S
Gause Institute of New Antibiotics, St. Bolshaya Pirogovskaya 11, 119021 Moscow, Russia.
Faculty of Biology, Lomonosov Moscow State University, 1-12 Leninskie Gory, 119234 Moscow, Russia.
Microorganisms. 2023 Oct 19;11(10):2587. doi: 10.3390/microorganisms11102587.
We have performed a characterization of cultivated haloalkalitolerant fungi from the sediments of Big Tambukan Lake in order to assess their biodiversity and antimicrobial activity. This saline, slightly alkaline lake is known as a source of therapeutic sulfide mud used in sanatoria of the Caucasian Mineral Waters, Russia. Though data on bacteria and algae observed in this lake are available in the literature, data on fungi adapted to the conditions of the lake are lacking. The diversity of haloalkalitolerant fungi was low and represented by ascomycetes of the genera , , , , , , , , , and . Most of the fungi were characterized by moderate alkaline resistance, and they tolerated NaCl concentrations up to 10% /. The analysis of the antimicrobial activity of fungi showed that 87.5% of all strains were active against , and 39.6% were also determined to be effective against . The majority of the strains were also active against and , about 66.7% and 62.5%, respectively. These studies indicate, for the first time, the presence of polyextremotolerant fungi in the sediments of Big Tambukan Lake, which probably reflects their involvement in the formation of therapeutic muds.
为了评估其生物多样性和抗菌活性，我们对大坦布坎湖沉积物中培养的耐盐碱真菌进行了表征。这个盐湖呈微碱性，是俄罗斯高加索矿泉疗养院所用治疗性硫化物泥浆的来源。尽管该湖中观察到的细菌和藻类数据在文献中已有记载，但适应该湖环境的真菌数据却很缺乏。耐盐碱真菌的多样性较低，以、、、、、、、、和属的子囊菌为代表。大多数真菌具有中等耐碱性，能耐受高达10%/的NaCl浓度。对真菌抗菌活性的分析表明，所有菌株中有87.5%对有活性，39.6%也被确定对有效。大多数菌株对和也有活性，分别约为66.7%和62.5%。这些研究首次表明，大坦布坎湖沉积物中存在多极端耐受真菌，这可能反映了它们参与了治疗性泥浆的形成。
