Takikawa M, Kikkawa H, Asai T, Yamaguchi N, Ishikawa D, Tanaka M, Ogino K, Taki T, Oku N
Department of Radiobiochemistry, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
FEBS Lett. 2000 Jan 28;466(2-3):381-4. doi: 10.1016/s0014-5793(00)01110-8.
GD1alpha ganglioside-replica peptides were recently isolated from a phage-displayed random pentadecapeptide library by assaying for inhibition of adhesion of RAW117-H10 lymphosarcoma cells to hepatic sinusoidal microvessel endothelial (HSE) cells. We show here that the Trp-His-Trp (WHW) peptide was identified as a minimal sequence of the GD1alpha-replica peptide WHWRHRIPLQLAAGR. The addition of WHW peptide-attached liposomes displayed efficient inhibition of liver metastasis of RAW117-H10 cells as well as of GD1alpha-mediated adhesion of RAW117-H10 cells to HSE cells in vitro. These results suggest that engineered liposomes for peptide delivery are applicable to treatment for metastasis.
GD1α神经节苷脂复制肽最近通过检测对RAW117 - H10淋巴肉瘤细胞与肝窦微血管内皮（HSE）细胞黏附的抑制作用，从噬菌体展示的随机十五肽文库中分离得到。我们在此表明，Trp - His - Trp（WHW）肽被鉴定为GD1α复制肽WHWRHRIPLQLAAGR的最小序列。添加附着有WHW肽的脂质体在体外可有效抑制RAW117 - H10细胞的肝转移以及GD1α介导的RAW117 - H10细胞与HSE细胞的黏附。这些结果表明，用于肽递送的工程化脂质体可应用于转移治疗。
