Yun Z, Smith T W, Menter D G, McIntire L V, Nicolson G L
Department of Tumor Biology, The University of Texas M. D. Anderson Cancer Center, Houston, USA.
Clin Exp Metastasis. 1997 Jan;15(1):3-11. doi: 10.1023/a:1018451616309.
RGD-containing substrates were used to study static and hydrodynamic adhesion of murine RAW117 large-cell lymphoma sublines with differential liver-metastatic potentials. Highly liver-metastatic RAW117-H10 cells had higher rates of static adhesion to vitronectin, fibronectin and (GRGDS)4 than poorly metastatic RAW117-P and moderately liver-metastatic RAW117-L17 cells. Under hydrodynamic conditions, adhesion stabilization was more rapid for H10 cells compared to P or L17 cells. Among the RGD peptides, only the polymeric RGD peptide (GRGDS)4 mediated strong static adhesion of H10 cells. Interestingly, all the RGD peptides mediated adhesion stabilization for H10 cells but still not for L17 or P cells under hydrodynamic conditions. Integrin alpha(v) beta3 was involved in stabilizing hydrodynamic adhesion to (GRGDS)4, monomeric RGD peptide R1, but was less important in static adhesion to monomeric RGD peptides. Differential adhesion to liver sinusoidal endothelial cell-derived extracellular matrix (H10 >> L17 > P) was observed under hydrodynamic but not static conditions. Integrin alpha(v) beta3 was also important in hydrodynamic adhesion to liver sinusoidal endothelial cell-derived extracellular matrix. We believe that strong static and hydrodynamic adhesion of H10 cells and their capability of altering adhesive behavior in response to fluid shear may contribute to liver metastasis.
含RGD的底物被用于研究具有不同肝转移潜能的小鼠RAW117大细胞淋巴瘤亚系的静态和流体动力学黏附。高肝转移潜能的RAW117-H10细胞对玻连蛋白、纤连蛋白和(GRGDS)4的静态黏附率高于低转移潜能的RAW117-P细胞和中度肝转移潜能的RAW117-L17细胞。在流体动力学条件下，与P细胞或L17细胞相比，H10细胞的黏附稳定更快。在RGD肽中，只有聚合RGD肽(GRGDS)4介导H10细胞的强静态黏附。有趣的是，在流体动力学条件下，所有RGD肽都介导H10细胞的黏附稳定，但对L17或P细胞则不然。整合素α(v)β3参与稳定对(GRGDS)4、单体RGD肽R1的流体动力学黏附，但在对单体RGD肽的静态黏附中不太重要。在流体动力学而非静态条件下观察到对肝窦内皮细胞衍生的细胞外基质的差异黏附(H10 >> L17 > P)。整合素α(v)β3在对肝窦内皮细胞衍生的细胞外基质的流体动力学黏附中也很重要。我们认为，H10细胞的强静态和流体动力学黏附及其响应流体剪切改变黏附行为的能力可能有助于肝转移。
