Penning M, Beld M, Goudsmit J
Department of Human Retrovirology, University of Amsterdam, Amsterdam, The Netherlands.
Virology. 2000 Mar 1;268(1):12-6. doi: 10.1006/viro.1999.0150.
Following reports of the finding of cDNA of RNA viruses in cells containing an endogenous retrovirus-encoded reverse transcriptase, we looked for the presence of hepatitis C virus (HCV) DNA in peripheral blood mononuclear cells (PBMC) of injecting drug users seropositive for both HCV and human immunodefiency virus (HIV). We tested serial PBMC samples from four HCV infected individuals; one was seronegative for HIV, two seroconverted for HIV during follow-up, and one was seropositive for HIV throughout the study period. HCV RNA was found in PBMC and plasma samples at all time points tested. Similarly, HIV RNA was found in all PBMC and plasma samples following HIV seroconversion. In contrast, no HCV DNA was detected in any PBMC sample, whereas HIV DNA was found in all tested PBMC samples following HIV seroconversion, indicative of active HIV reverse transcriptase in these PBMC samples. These results do not support the hypothesis that HCV viraemia is related to retrotranscription of the HCV RNA genome into DNA in peripheral blood mononuclear cells coinfected with HIV. The potential of HIV RT to retrotranscribe HCV RNA into DNA awaits studies of liver cells coinfected with HCV and HIV.
在有内源性逆转录病毒编码的逆转录酶的细胞中发现RNA病毒的cDNA的报道之后，我们在同时感染丙型肝炎病毒（HCV）和人类免疫缺陷病毒（HIV）的注射吸毒者的外周血单个核细胞（PBMC）中寻找HCV DNA的存在。我们检测了来自四名HCV感染个体的系列PBMC样本；一名个体HIV血清学阴性，两名个体在随访期间HIV血清阳转，一名个体在整个研究期间HIV血清学阳性。在所有检测时间点的PBMC和血浆样本中均检测到HCV RNA。同样，在HIV血清阳转后的所有PBMC和血浆样本中均检测到HIV RNA。相比之下，在任何PBMC样本中均未检测到HCV DNA，而在HIV血清阳转后的所有检测PBMC样本中均发现了HIV DNA，这表明这些PBMC样本中有活跃的HIV逆转录酶。这些结果不支持HCV病毒血症与在同时感染HIV的外周血单个核细胞中将HCV RNA基因组逆转录为DNA有关的假说。HIV逆转录酶将HCV RNA逆转录为DNA的可能性有待对同时感染HCV和HIV的肝细胞进行研究。
