Ademoğlu E, Gökkuşu C, Palandüz S
Department of Biochemistry, Faculty of Medicine, University of Istanbul, Turkey.
Int J Vitam Nutr Res. 2000 Jan;70(1):3-7. doi: 10.1024/0300-9831.70.1.3.
It has been shown that the lipid composition of plasma membrane can be modified in vivo by dietary fat. It has also been observed that an increase in the cholesterol content of plasma membranes results in decreased activities of ATPases. In the present study, we evaluated the changes in the activities of ATPases from erythrocytes, hepatocytes, and kidney cortex caused by cholesterol-rich diet in rats and subsequently examined the role of vitamin E administration on the cholesterol-induced effects in these tissues. Administration of hypercholesterolemic diet to the rats for 4.5 months, significantly decreased membrane Na(+)-K(+)-ATPase and Ca+2-ATPase activities in comparison to the controls in all tissues studied. Vitamin E supplementation to the hypercholesterolemic rats led to a recovery in membrane ATPase activities. In conclusion, vitamin E supplementation to the rats provided protection against hypercholesterolemic diet-induced impairment of membrane-bound ATPases.
研究表明，饮食中的脂肪可在体内改变质膜的脂质组成。还观察到质膜胆固醇含量的增加会导致ATP酶活性降低。在本研究中，我们评估了富含胆固醇的饮食对大鼠红细胞、肝细胞和肾皮质中ATP酶活性的影响，随后研究了给予维生素E对这些组织中胆固醇诱导效应的作用。给大鼠喂食高胆固醇饮食4.5个月后，与所有研究组织中的对照组相比，膜Na(+)-K(+)-ATP酶和Ca+2-ATP酶活性显著降低。给高胆固醇血症大鼠补充维生素E可使膜ATP酶活性恢复。总之，给大鼠补充维生素E可预防高胆固醇饮食引起的膜结合ATP酶损伤。
