Stanely Mainzen Prince P, Karthick M
Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar 608 002, Tamil Nadu, India.
J Biochem Mol Toxicol. 2007;21(1):1-6. doi: 10.1002/jbt.20151.
The present study was designed to evaluate the preventive role of rutin on lipids, lipoproteins, and ATPases in normal and isoproterenol (ISO)-induced myocardial infarction in rats. Rutin (40 and 80 mg/kg) was orally administered to rats for a period of 42 days. After that period, isoproterenol (150 mg/kg) was injected subcutaneously to male wistar rats at an interval of 24 h for 2 days. The weight of heart and the concentrations of total cholesterol, triglycerides, and free fatty acids were increased significantly (p < 0.05), and the concentration of phospholipids was decreased significantly (p < 0.05) in the heart of ISO-treated rats. ISO-treated rats also showed a significant increase (p < 0.05) in the levels of total cholesterol, triglycerides, phospholipids, low-density lipoprotein cholesterol (LDL-C), and very low-density lipoprotein cholesterol (VLDL-C) with a significant decrease (p < 0.05) in high-density lipoprotein cholesterol (HDL-C) level in serum. The activities of sodium potassium dependent adenosine triphosphatase (Na(+)/K(+) ATPase) and magnesium-dependent adenosine triphosphatase (Mg(2+) ATPase) were decreased significantly (p < 0.05), and the activity of calcium-dependent adenosine triphosphatase (Ca(2+)ATPase) was increased significantly (p < 0.05) in the heart in ISO-treated rats. Pretreatment with rutin at doses of 40 or 80 mg/kg to ISO-treated rats showed a significant (p < 0.05) effect in all the parameters studied. Oral administration of rutin to normal rats did not show any significant effect. Thus, the results of our study show that pretreatment with rutin maintained the levels of lipids, lipoproteins, and ATPases in ISO-induced myocardial infarcted rats. The observed effects might be due to the antioxidant potential of rutin.
本研究旨在评估芦丁对正常大鼠以及异丙肾上腺素（ISO）诱导的大鼠心肌梗死模型中脂质、脂蛋白和ATP酶的预防作用。将芦丁（40和80毫克/千克）口服给予大鼠，持续42天。在此期间过后，以24小时的间隔皮下注射异丙肾上腺素（150毫克/千克）给雄性Wistar大鼠，共注射2天。ISO处理组大鼠心脏重量以及心脏中总胆固醇、甘油三酯和游离脂肪酸的浓度显著增加（p < 0.05），而磷脂浓度显著降低（p < 0.05）。ISO处理组大鼠血清中总胆固醇、甘油三酯、磷脂、低密度脂蛋白胆固醇（LDL-C）和极低密度脂蛋白胆固醇（VLDL-C）水平也显著升高（p < 0.05），高密度脂蛋白胆固醇（HDL-C）水平显著降低（p < 0.05）。ISO处理组大鼠心脏中钠钾依赖性三磷酸腺苷酶（Na(+)/K(+) ATP酶）和镁依赖性三磷酸腺苷酶（Mg(2+) ATP酶）的活性显著降低（p < 0.05），而钙依赖性三磷酸腺苷酶（Ca(2+)ATP酶）的活性显著升高（p < 0.05）。以40或80毫克/千克剂量的芦丁对ISO处理组大鼠进行预处理，在所研究的所有参数中均显示出显著（p < 0.05）效果。对正常大鼠口服芦丁未显示出任何显著影响。因此，我们的研究结果表明，用芦丁预处理可维持ISO诱导的心肌梗死大鼠中脂质、脂蛋白和ATP酶的水平。观察到的这些效果可能归因于芦丁的抗氧化潜力。
