关于血红素缺乏的兔网织红细胞裂解物中蛋白质合成延迟抑制的机制
On the mechanism of delayed inhibition of protein synthesis in heme-defecient rabbit reticulocyte lysates.
作者信息
Cherbas L, London I M
出版信息
Proc Natl Acad Sci U S A. 1976 Oct;73(10):3506-10. doi: 10.1073/pnas.73.10.3506.
Abstract
In the absence of added hemin, protein synthesis in a rabbit reticulocyte lysate declines abruptly (shuts off) after about 5 min at 30 degrees. In these studies we have examined the basis for the lag period preceding shut-off. The initiation factor that binds Met-tRNAf, previously shown to be rate-limiting in inhibited, heme-deficient lysates, is found to be used stoichiometrically in the presence of excess inhibitor. We suggest that a principal effect of the inhibitor is to impair the recycling of the Met-tRNAf-binding factor; the lag period is attributable largely to the presence of a pool of excess Met-tRNAf-binding factor, which, once used in initiation, cannot be recycled because of the action of the inhibitor.
摘要
在没有添加血红素的情况下，兔网织红细胞裂解物中的蛋白质合成在30℃下约5分钟后会突然下降（停止）。在这些研究中，我们研究了停止前延迟期的基础。先前已证明在受抑制的、血红素缺乏的裂解物中结合Met-tRNAf的起始因子是限速因子，发现在存在过量抑制剂的情况下，它按化学计量被使用。我们认为抑制剂的主要作用是损害Met-tRNAf结合因子的再循环；延迟期很大程度上归因于过量Met-tRNAf结合因子池的存在，一旦在起始过程中被使用，由于抑制剂的作用就无法再循环。
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