血红素控制的蛋白质合成抑制剂的作用模式：对来自兔网织红细胞的因子的研究。
Mode of action of the hemin-controlled inhibitor of protein synthesis: studies with factors from rabbit reticulocytes.
作者信息
de Haro C, Ochoa S
出版信息
Proc Natl Acad Sci U S A. 1978 Jun;75(6):2713-6. doi: 10.1073/pnas.75.6.2713.
Abstract
Previously [de Haro, C., Datta, A & Ochoa, S. (1978) Proc. Natl. Acad. Sci. USA 75, 243--247] it was shown with initiation factors from Artemia salina embryos that the activity of the initiator methionyl-tRNA binding factor eIF-2 is stimulated by another factor (ESP, for eIF-2 stimulating protein) present, like eIF-2, in ribosomal salt washes. Incubation of eIF-2 with translational inhibitor from rabit reticulocytes, in the presence of ATP, abolished the ESP effect. At physiological concentrations eIF-2 was virtually inactive without ESP. These observations indicated that the translational inhibitor acts by converting eIF-2 to a form that is not stimulated by ESP. The same observations have now been made with eIF-2 and ESP from rabbit reticulocytes but, in this case, the dependence of eIF-2 activity on ESP is much more pronounced than with the A. salina factors. eIF-2 from reticulocytes interacts with ESP from A. salina and conversely.
摘要
先前[德·哈罗，C.，达塔，A和奥乔亚，S.（1978年）《美国国家科学院院刊》75，243 - 247]用卤虫胚胎中的起始因子表明，起始甲硫氨酰 - tRNA结合因子eIF - 2的活性受到另一种因子（ESP，即eIF - 2刺激蛋白）的刺激，该因子与eIF - 2一样存在于核糖体盐洗物中。在ATP存在的情况下，将eIF - 2与兔网织红细胞的翻译抑制剂一起孵育，消除了ESP的作用。在生理浓度下，没有ESP时eIF - 2实际上是无活性的。这些观察结果表明，翻译抑制剂通过将eIF - 2转化为不受ESP刺激的形式起作用。现在用兔网织红细胞的eIF - 2和ESP也得到了相同的观察结果，但在这种情况下，eIF - 2活性对ESP的依赖性比卤虫因子更为明显。网织红细胞的eIF - 2与卤虫的ESP相互作用，反之亦然。
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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On the mechanism of delayed inhibition of protein synthesis in heme-defecient rabbit reticulocyte lysates.关于血红素缺乏的兔网织红细胞裂解物中蛋白质合成延迟抑制的机制
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Polypeptide chain initiation in eukaryotes: mechanism of formation of initiation complex.真核生物中的多肽链起始：起始复合物的形成机制。
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Protein synthesis in rabbit reticulocytes. XV. Isolation of a ribosomal protein factor (CO-EIE-1) which stimulates Met-tRNAfMet binding to EIF-1.
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Additional evidence that the hemin-controlled translational repressor from rabbit reticulocytes is a protein kinase.来自兔网织红细胞的血红素控制的翻译阻遏物是一种蛋白激酶的更多证据。
Biochem Biophys Res Commun. 1977 Jan 24;74(2):559-69. doi: 10.1016/0006-291x(77)90340-0.
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Phosphorylation of initiation factor elF-2 and the control of reticulocyte protein synthesis.起始因子elF-2的磷酸化与网织红细胞蛋白质合成的调控
Cell. 1977 May;11(1):187-200. doi: 10.1016/0092-8674(77)90330-0.
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Mode of action of the hemin-controlled inhibitor of protein synthesis.血红素控制的蛋白质合成抑制剂的作用模式。
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8
Binding and release of eukaryotic initiation factor eIF-2 and GTP during protein synthesis initiation.蛋白质合成起始过程中真核生物起始因子eIF-2与GTP的结合及释放
Proc Natl Acad Sci U S A. 1978 Jan;75(1):204-8. doi: 10.1073/pnas.75.1.204.
9
Regulation of protein synthesis in reticulocyte lysates: phosphorylation of methionyl-tRNAf binding factor by protein kinase activity of translational inhibitor isolated from hemedeficient lysates.网织红细胞裂解物中蛋白质合成的调控：从血红素缺乏的裂解物中分离出的翻译抑制剂的蛋白激酶活性使甲硫氨酰 - tRNAf结合因子磷酸化。
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Specificity of the protein kinase activity associated with the hemin-controlled repressor of rabbit reticulocyte.与兔网织红细胞血红素控制阻遏物相关的蛋白激酶活性的特异性
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