Chen L B, Gallimore P H, McDougall J K
Proc Natl Acad Sci U S A. 1976 Oct;73(10):3570-4. doi: 10.1073/pnas.73.10.3570.
The distribution on the cell surface of the large external LETS protein that is transformation sensitive of normal, transformed and tumorigenic cells was examined by immunofluorescent staining. A correlation was established between the expression of fibril-like LETS protein and the oncogenic capabilities of a series of adenovirus-transformed cell lines. In cells expressing a transformed phenotype in vitro, LETS protein is only detected in cell-cell contact areas, wheras in "untransformed" cells LETS protein is distributed over the cell surface. Transformed cells capable of inducing invasive tumors, and the cells of established tumor lines, have low or undetectable levels of LETS protein, as measured by this method. The results indicate that LETS protein has a role in cell-cell adhesion and that reduced expression of this protein at the cell surface is related to the oncogenic phenotype. This relationship has been established for experimentally induced and spontaneous tumors.
通过免疫荧光染色检查了正常、转化和致瘤细胞中对转化敏感的大型细胞外LETS蛋白在细胞表面的分布。在一系列腺病毒转化细胞系中，纤维状LETS蛋白的表达与致癌能力之间建立了相关性。在体外表达转化表型的细胞中，LETS蛋白仅在细胞-细胞接触区域被检测到，而在“未转化”细胞中，LETS蛋白分布在细胞表面。用这种方法测量，能够诱导侵袭性肿瘤的转化细胞以及已建立肿瘤系的细胞，其LETS蛋白水平较低或无法检测到。结果表明，LETS蛋白在细胞-细胞黏附中起作用，并且该蛋白在细胞表面的表达降低与致癌表型有关。这种关系已在实验诱导的肿瘤和自发肿瘤中得到证实。
