绵羊小肠腺癌细胞的培养生长
Growth in culture of adenocarcinoma cells from the small intestine of sheep.
作者信息
Norval M, Head K W, Else R W, Hart H, Neill W A
出版信息
Br J Exp Pathol. 1981 Jun;62(3):270-82.
Explant cultures were initiated from adenocarcinoma of the small intestine in sheep and from various metastases. Several cell types grew, most being fibroblastic in nature. However, 2 cultures yielded mixed cells which arranged themselves into areas of epithelial-like cells surrounded by fibroblast-like cells and this pattern was consistent over 30 subcultures and several months of culturing. The epithelial-like cells were separated from the others by the use of a modified medium containing citrulline or by sedimentation through a bovine serum albumin solution. Various properties, including their growth rate in 5% and 0.5% serum, the absence of surface fibronectin and their ability to grow in semi-solid agar, indicated that they may represent carcinoma cells. Screening for virus production from these cells and all other explant cultures proved negative.
外植体培养源自绵羊小肠腺癌及各种转移灶。有几种细胞类型生长出来，大多数本质上是成纤维细胞样。然而，有2种培养物产生了混合细胞，这些细胞自行排列成上皮样细胞区域，周围是成纤维细胞样细胞，这种模式在30次传代培养和数月的培养过程中保持一致。通过使用含有瓜氨酸的改良培养基或通过牛血清白蛋白溶液沉降，将上皮样细胞与其他细胞分离。各种特性，包括它们在5%和0.5%血清中的生长速率、表面纤连蛋白的缺失以及它们在半固体琼脂中生长的能力，表明它们可能代表癌细胞。对这些细胞和所有其他外植体培养物进行病毒产生的筛查结果均为阴性。
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