Willard M B
Proc Natl Acad Sci U S A. 1976 Oct;73(10):3641-5. doi: 10.1073/pnas.73.10.3641.
One of the polypeptides (H1) of the rabbit nervous system occurs in an altered form (H2) in some rabbits. The electrophoretic mobility of H2 on sodium dodecyl sulfate-polyacrylamide gels is about 6% greater than that of H1, suggesting that the two polypeptides differ in molecular weight by about 10,000. The alteration is genetically determined since (i) rabbit phenotypes corresponding to all possible genotypes (H1H1, H1H2, H2H2) were present in an outbred population of rabbits, (ii) the frequency of the phenotype corresponding to the heterozygous genotype (H1H2) was smaller in partially inbred rabbit populations than in outbred populations, and (iii) all of the individuals examined from two partially inbred strains (WH/J and X/J) were of the phenotype that would be expected if they were homozygous (H2H2) for the rare allele of the gene. Preliminary evidence indicates that this polymorphic polypeptide is most abundant in the white matter of the nervous systems of several mammalian species, and is distributed with buffers of low ionic strength and centrifuged at 100,000 Xg.
兔神经系统的一种多肽（H1）在一些兔子中以一种改变的形式（H2）出现。H2在十二烷基硫酸钠-聚丙烯酰胺凝胶上的电泳迁移率比H1大约高6%，这表明这两种多肽的分子量相差约10,000。这种改变是由基因决定的，因为（i）在一个远交兔群中存在对应于所有可能基因型（H1H1、H1H2、H2H2）的兔表型，（ii）在部分近交兔群中，对应杂合基因型（H1H2）的表型频率比远交群体中的小，并且（iii）从两个部分近交品系（WH/J和X/J）中检查的所有个体都具有如果它们对于该基因的稀有等位基因为纯合（H2H2）时所预期的表型。初步证据表明，这种多态性多肽在几种哺乳动物物种神经系统的白质中最为丰富，并且在低离子强度缓冲液中分布，并在100,000×g下离心。
