Willard M, Cowan W M, Vagelos P R
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2183-7. doi: 10.1073/pnas.71.6.2183.
Using a method of gradient gel electrophoresis coupled with autoradiography, we have analyzed the polypeptide composition of the proteins being transported down the axons of the projecting neurons of the rabbit retina. This analysis reveals: (1) the molecular weight distribution of 43 transported polypeptides; (2) the existence of at least four components of intra-axonal protein transport, each characterized by an unique polypeptide composition as well as by an unique velocity of transport; (3) the disappearance of individual labeled intra-axonally transported proteins from the axons and synaptic terminals with half-times ranging from several hours to more than 8 days. The possibility that intra-axonal transport functions as a temporal mediator of alterations in axonal or synaptic events is discussed in relation to these findings.
我们采用梯度凝胶电泳结合放射自显影的方法，分析了沿兔视网膜投射神经元轴突下行运输的蛋白质的多肽组成。该分析揭示：（1）43种运输多肽的分子量分布；（2）轴突内蛋白质运输至少存在四个组分，每个组分具有独特的多肽组成以及独特的运输速度；（3）轴突和突触终末中单个标记的轴突内运输蛋白消失，其半衰期从数小时到超过8天不等。结合这些发现，讨论了轴突内运输作为轴突或突触事件变化的时间介导因子的可能性。
