怀孕和外源性类固醇治疗可调节大鼠子宫中舒张性EP(2)受体和收缩性FP受体的表达。
Pregnancy and exogenous steroid treatments modulate the expression of relaxant EP(2) and contractile FP receptors in the rat uterus.
作者信息
Dong Y L, Yallampalli C
机构信息
Department of Obstetrics and Gynecology, The University of Texas Medical Branch, Galveston, Texas 77555, USA.
出版信息
Biol Reprod. 2000 Mar;62(3):533-9. doi: 10.1095/biolreprod62.3.533.
Prostaglandins (PGs) interact with specific receptors on plasma membranes to regulate myometrial activity in many species. The present study examined whether the expression of relaxant prostaglandin E receptor subtype two (EP(2)) and contractile prostaglandin F receptor (FP) mRNA in the rat uterus is changed during various states of pregnancy and regulated by steroid hormones. Expression of mRNA for EP(2) and FP receptors in the full-thickness uteri was analyzed by reverse transcription-polymerase chain reaction using specific primers. Abundance of receptor mRNA was expressed relative to beta-actin mRNA. Results showed that 1) mRNA for EP(2) receptors in the rat uterus was substantially increased during pregnancy (320%) compared with the nonpregnant state (100%, P < 0.01), and declined during labor at term (36% vs. 100% in control, P < 0.01); 2) mRNA expression for FP receptors in rat uterus was increased during pregnancy (333% vs. 100% in nonpregnant rats, P < 0. 01) and reached maximal levels during labor (515% vs. 100% in control, P < 0.01); 3) upon RU-486 treatment on Day 19 of pregnancy, uterine EP(2) receptor mRNA levels were decreased (18% vs. 100% in control, P < 0.01), and FP mRNA levels were increased (357% vs. 100% in control, P < 0.01); 4) with ICI 164384 (an antiestrogen) treatment on Day 19 of gestation, uterine FP receptor mRNA levels were decreased without effects on EP(2) receptors; 5) in ovariectomized (ovx) rats, progesterone increased EP(2) (163% vs. 100% in control, P < 0.01) and had no effects on FP receptor mRNA expression in the rat uterus; 6) estradiol increased FP receptor mRNA levels (358% vs. 100% in control, P < 0.01) and had no effects on EP(2) mRNA in the ovx rat uterus. Therefore, we conclude that steroid hormones modulate the mRNA for relaxant EP(2) and contractile FP receptors for PGs in the uterus and thus regulate uterine activity during pregnancy and labor.
前列腺素（PGs）与质膜上的特定受体相互作用，以调节许多物种的子宫肌层活性。本研究检测了大鼠子宫中松弛性前列腺素E受体亚型二（EP(2)）和收缩性前列腺素F受体（FP）mRNA的表达在妊娠不同阶段是否发生变化以及是否受甾体激素调节。使用特异性引物通过逆转录-聚合酶链反应分析全层子宫中EP(2)和FP受体的mRNA表达。受体mRNA丰度相对于β-肌动蛋白mRNA进行表达。结果显示：1）与未妊娠状态（100%）相比，大鼠子宫中EP(2)受体的mRNA在妊娠期间大幅增加（320%，P<0.01），足月分娩时下降（36% vs.对照的100%，P<0.01）；2）大鼠子宫中FP受体的mRNA表达在妊娠期间增加（333% vs.未妊娠大鼠的100%，P<0.01），并在分娩时达到最高水平（515% vs.对照的100%，P<0.01）；3）在妊娠第19天用RU-486处理后，子宫EP(2)受体mRNA水平降低（18% vs.对照的100%，P<0.01），而FP mRNA水平升高（357% vs.对照的100%，P<0.01）；4）在妊娠第19天用ICI 164384（一种抗雌激素）处理后，子宫FP受体mRNA水平降低，对EP(2)受体无影响；5）在去卵巢（ovx）大鼠中，孕酮增加了EP(2)（163% vs.对照的100%，P<0.01），对大鼠子宫中FP受体mRNA表达无影响；6）雌二醇增加了ovx大鼠子宫中FP受体mRNA水平（358% vs.对照的100%，P<0.01），对EP(2) mRNA无影响。因此，我们得出结论，甾体激素调节子宫中PGs的松弛性EP(2)和收缩性FP受体的mRNA，从而在妊娠和分娩期间调节子宫活性。
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