Helgeland K
Scand J Dent Res. 1976 Sep;84(5):276-85. doi: 10.1111/j.1600-0722.1976.tb00490.x.
The time course for incorporation of KC-proline into various fractions of rabbit dental pulp in vitro has been measured. In the TCA-soluble precursor pool a steady state level of activity was indicated upon incubation after 3 h, whereas incorporation into protein and 14C-hydroxyproline, i.e. collagen formation, increased linearly for 9 h, leveling off upon further incubation. A lag period of about 3 h was indicated for the appearance of high molecular weight 14C-activity, including 14C-hydroxyproline, in the medium, increasing linearly from 3 h to the end of the incubation period (22 h). In this system, fluoride exhibited a dose-dependent inhibitory effect. At 5.3 mM fluoride the uptake of 14C-proline into the TCA-soluble pool was inhibited by about 50%, and the incorporation into protein and the subsequent conversion to hydroxyproline by about 90 and 60%, respectively. Release of collagen, i.e. 14C-hydroxyproline-containing material, seemed to be the process most sensitive to fluoride; it was inhibited by about 50% at the lowest concentration (1.3 mM) tested.
已测定了体外培养时兔牙髓不同组分中KC - 脯氨酸的掺入时间进程。在三氯乙酸（TCA）可溶性前体池中，孵育3小时后活性达到稳定状态水平，而掺入蛋白质和14C - 羟脯氨酸（即胶原蛋白形成）的量在9小时内呈线性增加，进一步孵育后趋于平稳。在培养基中，高分子量14C活性（包括14C - 羟脯氨酸）的出现有大约3小时的延迟期，从3小时到孵育期结束（22小时）呈线性增加。在该系统中，氟化物表现出剂量依赖性抑制作用。在5.3 mM氟化物时，14C - 脯氨酸进入TCA可溶性池的摄取被抑制约50%，掺入蛋白质以及随后转化为羟脯氨酸的过程分别被抑制约90%和60%。胶原蛋白（即含14C - 羟脯氨酸的物质）的释放似乎是对氟化物最敏感的过程；在测试的最低浓度（1.3 mM）下，其释放被抑制约50%。
