Uitto V J, Antila R, Ranta R
Acta Odontol Scand. 1975;33(5):287-98. doi: 10.3109/00016357509004634.
The aim of the study was to observe pulpal collagen synthesis in response to trauma and to glucocorticoid medication. The material consisted of 290 rabbit pulps and 76 human premolar pulps. Collagen synthesis was determined by incubating whole pulps in a medium containing [14C]proline, and measuring the formation of [14C]hydroxyproline. The effect of glucocorticoids was studied in vitro using rabbit pulps. Hydrocortisone and dexamethasone inhibited collagen synthesis, whereas prednisolone had no marked effect. Hydrocortisone was found to inhibit the synthesis of [14C]hydroxyproline in neutral salt soluble and insoluble non-dialyzable collagen fractions. [14C]hydroxyproline in the dialyzable fraction was increased, suggesting that hydrocortisone increased collagen degradation. In the human material, premolar pulps were experimentally exposed and then medicated with capping agents. The contralateral teeth were exposed and capped with other capping materials, in some cases they were left as intact controls. The exposure led to an increase in the collagen synthesis as indicated by increased [14C]hydroxyproline formation and elevated protocollagen proline hydroxylase activity in the pulp. This enzyme activity was suppressed in pulps capped with a glucocorticoid paste. In addition, the collagen synthesis rate was lower in pulps treated with another glucocorticoid containing compound, when compared to pulps capped with a calcium hydroxide preparation.
本研究的目的是观察牙髓胶原合成对创伤和糖皮质激素药物治疗的反应。材料包括290个兔牙髓和76个人类前磨牙牙髓。通过将整个牙髓在含有[14C]脯氨酸的培养基中孵育，并测量[14C]羟脯氨酸的形成来确定胶原合成。使用兔牙髓在体外研究糖皮质激素的作用。氢化可的松和地塞米松抑制胶原合成，而泼尼松龙没有显著作用。发现氢化可的松抑制中性盐可溶性和不可透析性胶原组分中[14C]羟脯氨酸的合成。可透析组分中的[14C]羟脯氨酸增加，表明氢化可的松增加了胶原降解。在人类材料中，前磨牙牙髓经实验性暴露，然后用盖髓剂治疗。对侧牙齿暴露并用其他盖髓材料覆盖，在某些情况下将其作为完整对照。如牙髓中[14C]羟脯氨酸形成增加和原胶原脯氨酸羟化酶活性升高所示，暴露导致胶原合成增加。用糖皮质激素糊剂覆盖的牙髓中这种酶活性受到抑制。此外，与用氢氧化钙制剂覆盖的牙髓相比，用另一种含糖皮质激素的化合物处理的牙髓中胶原合成速率较低。
