Martinez M, Martinez F E, Garcia P J, Cagnon V H, Mello Júnior W, Padovani C R
Department of Morphology and Pathology, Federal University of São Carlos, Brazil.
J Submicrosc Cytol Pathol. 1999 Oct;31(4):469-75.
The effects of alcohol ingestion on the epithelial layer of the uterine endometrium of rats submitted to experimental chronic alcoholism were observed by morphometric methods. Sixty adult rats (Rattus norvegicus albinus) of the same age (3 months) and weighing on average 228 g were divided into two groups. The control group received solid diet, rat chow, and tap water ad libitum. The experimental group received the same solid diet and was allowed to drink only sugar cane brandy dissolved in 30 degrees Gay Lussac (v/v). At the end of 90, 180 and 270 days of treatment, the animals at estro were anaesthetized with ethyl ether, weighed and sacrificed. The internal reproductive organs were dissected, weighed and fixed. The final mean body weights were similar in the control and alcoholic groups. The histological results showed intense atrophy of the lining epithelium of the endometrium of uterine horns in the alcoholic group. Morphometric analysis confirmed the endometrial epithelial atrophy, showing that the alcoholic group had small cytoplasmic and nuclear areas and small cytoplasmic and nuclear perimeter compared to the control group. Scanning electron microscopic images showed intense lipid droplets accumulation in the epithelial cells from alcoholic group. Therefore, we concluded that alcohol acts as a toxin on the epithelial layer of the rat endometrium.
通过形态计量学方法观察了酒精摄入对实验性慢性酒精中毒大鼠子宫内膜上皮层的影响。将60只年龄相同（3个月）、平均体重228克的成年大鼠（白化挪威大鼠）分为两组。对照组随意给予固体饲料、大鼠饲料和自来水。实验组给予相同的固体饲料，只允许饮用溶解在30度盖·吕萨克酒精度（体积/体积）中的甘蔗白兰地。在治疗90、180和270天结束时，处于动情期的动物用乙醚麻醉、称重并处死。解剖、称重并固定其内部生殖器官。对照组和酒精组的最终平均体重相似。组织学结果显示，酒精组子宫角内膜的衬里上皮出现严重萎缩。形态计量学分析证实了子宫内膜上皮萎缩，表明与对照组相比，酒精组的细胞质和细胞核面积较小，细胞质和细胞核周长也较小。扫描电子显微镜图像显示，酒精组的上皮细胞中有大量脂滴积累。因此，我们得出结论，酒精对大鼠子宫内膜上皮层具有毒性作用。
