Martinez M, Mattos E C, Mello W J, Cagnon V H, Martinez F E
Department of Morphology and Pathology, Federal University of São Carlos, SP, Brazil.
J Submicrosc Cytol Pathol. 2001 Oct;33(4):453-61.
The objective of the present study was to assess the possible toxic effects of chronic alcohol ingestion on the ultrastructure of the lining epithelium of the seminal vesicle of the rodent Calomys callosus, in order to contribute to the understanding of the consequences of alcohol abuse for the morphology of the male reproductive apparatus. Twenty six adult animals aged three months were divided into two experimental groups. The control group received a solid diet (Purina chow) and tap water, and the alcoholic group received the same solid diet and ethanol P.A. diluted 20% in water (v/v). After 210 days of treatment, all animals were anaesthetised, weighed and sacrificed. At the end of treatment, mean body and seminal vesicle weight did not differ between control and alcoholic animals. The epithelial cells of the alcoholic group showed many alterations such as the presence of lipid droplets of different sizes, nuclei with increased peripheral chromatin and greater electrondensity, megamitochondria, and intense dilatation of the cisterns of the granular endoplasmic reticulum. It was concluded that 20% ethanol provokes marked ultrastructural lesions in the seminal vesicle.
本研究的目的是评估长期摄入酒精对啮齿动物卡洛mys callosus精囊衬里上皮超微结构的可能毒性作用，以便有助于理解酒精滥用对雄性生殖器官形态的影响。将26只三个月大的成年动物分为两个实验组。对照组接受固体饮食（普瑞纳饲料）和自来水，酒精组接受相同的固体饮食和在水中按体积比20%稀释的分析纯乙醇（v/v）。治疗210天后，所有动物均被麻醉、称重并处死。治疗结束时，对照组和酒精组动物的平均体重和精囊重量没有差异。酒精组的上皮细胞显示出许多改变，如存在不同大小的脂滴、外周染色质增加且电子密度更大的细胞核、巨型线粒体以及颗粒内质网池的强烈扩张。得出的结论是，20%的乙醇会引发精囊明显的超微结构损伤。
