Colitz C M, Malarkey D, Dykstra M J, McGahan M C, Davidson M G
Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh 27606, USA.
Am J Vet Res. 2000 Feb;61(2):139-43. doi: 10.2460/ajvr.2000.61.139.
To determine histologic and immunohistochemical characteristics of the multifocal adherent plaques that commonly develop on the internal surfaces of the anterior and posterior lens capsules in dogs with cataracts.
31 anterior and 4 posterior capsular specimens collected during lens extraction surgery in dogs with cataracts.
Specimens were evaluated, using light and transmission electron microscopy. Immunohistochemical techniques were used to localize cytokeratin, vimentin, alpha-smooth muscle-specific actin, fibronectin, tenascin, and transforming growth factor-beta (TGF-beta) within plaques.
Histologically, plaques comprised elongated spindle-shaped cells that formed a placoid mass. Cells were embedded in an extracellular matrix containing collagen fibrils, often with duplicated or split basement membranes. Immunohistochemically, normal lens epithelial cells and cells within plaques stained for vimentin. Most cells and some areas of the extracellular matrix within plaques stained for TGF-beta and alpha-smooth muscle-specific actin. Fibronectin and tenascin were also detected in the extracellular matrix.
Canine lens capsular plaques are histologically and immunohistochemically similar to posterior capsule opacification and subcapsular cataracts in humans, which suggests that the canine condition, like the human conditions, is associated with fibrous metaplasia of lens epithelial cells. Transforming growth factor-beta may play a role in the genesis of capsular plaques. Because severity of plaques was correlated with stage of cataract development, earlier surgical removal of cataracts may be useful to avoid complications associated with plaque formation.
确定白内障犬眼前、后囊膜内表面常见的多灶性粘连斑块的组织学和免疫组化特征。
在白内障犬晶状体摘除手术中收集的31个前囊膜和4个后囊膜标本。
使用光学显微镜和透射电子显微镜对标本进行评估。采用免疫组化技术在斑块内定位细胞角蛋白、波形蛋白、α-平滑肌特异性肌动蛋白、纤连蛋白、腱生蛋白和转化生长因子-β（TGF-β）。
组织学上，斑块由形成板状团块的细长梭形细胞组成。细胞嵌入含有胶原纤维的细胞外基质中，常伴有重复或分裂的基底膜。免疫组化显示，正常晶状体上皮细胞和斑块内的细胞波形蛋白染色阳性。斑块内的大多数细胞和细胞外基质的一些区域TGF-β和α-平滑肌特异性肌动蛋白染色阳性。在细胞外基质中也检测到纤连蛋白和腱生蛋白。
犬晶状体囊膜斑块在组织学和免疫组化上与人类后囊膜混浊和囊下白内障相似，这表明犬的这种情况与人类情况一样，与晶状体上皮细胞的纤维化生有关。转化生长因子-β可能在囊膜斑块的发生中起作用。由于斑块的严重程度与白内障发展阶段相关，早期手术摘除白内障可能有助于避免与斑块形成相关的并发症。
