Gerardi J.G., Colitz C.M.H., Dubielzig R.R., Davidson M.G.
Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA; Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA; Department of Pathobiological Sciences, University of Wisconsin, Madison, WS, USA; Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.
Vet Ophthalmol. 1999;2(3):163-168. doi: 10.1046/j.1463-5224.1999.00071.x.
The objective of the study was to characterize the morphologic and immunohistochemical features of lens epithelial-derived proliferative membranes from the anterior segment of canine globes. These features were correlated with those previously identified for diseases resulting from lens epithelial cell (LEC) proliferation including posterior capsular opacification, traumatic subcapsular cataract, and subcapsular plaques associated with hypermature cataracts. Sixteen canine globes were removed as a result of glaucoma or other complications following cataract extraction. Light microscopic and immunohistochemical analysis was performed on sections from formalin-fixed, paraffin-embedded globes. The tissues were stained with a variety of antibodies for cellular markers for LECs, growth factors or other cellular constituents relevant to cellular metaplasia and proliferation. The membranes were composed of monolayers or multilayers of spindle-shaped cells on the external surfaces of the anterior and posterior lens capsule, ciliary processes, iris leaflets, and iridocorneal angle, and they could be seen extending from an obvious monolayer of LEC within the capsular sac. Variably, scattered pigment cells, presumably of uveal origin, were concurrently present. Cellular components of the membranes stained positive for vimentin, transforming growth factor-beta, basic fibroblast growth factor, and smooth muscle actin. An amorphous eosinophilic extracellular matrix consisting predominately of collagen was associated with the membranes. Proliferative anterior segment membranes following cataract surgery were morphologically and immunohistochemically similar to cellular and matrix components of posterior capsular opacification and capsular plaques seen with hypermature cataracts, both of which result from metaplasia and proliferation of LEC. The presence of these LEC-derived membranes in association with secondary glaucoma suggests that exuberant proliferation of LEC outside the confines of the lens capsular sac may cause pathologic alterations in the eye following cataract surgery in the dog.
本研究的目的是描述犬眼球前段晶状体上皮衍生的增殖性膜的形态学和免疫组织化学特征。这些特征与先前确定的由晶状体上皮细胞（LEC）增殖引起的疾病相关特征进行了关联，这些疾病包括后囊膜混浊、外伤性囊下白内障以及与过熟期白内障相关的囊下斑块。由于青光眼或白内障摘除术后的其他并发症，摘除了16只犬的眼球。对福尔马林固定、石蜡包埋的眼球切片进行了光学显微镜和免疫组织化学分析。用多种针对LEC细胞标志物、生长因子或与细胞化生和增殖相关的其他细胞成分的抗体对组织进行染色。这些膜由位于晶状体前、后囊膜、睫状突、虹膜小叶和虹膜角膜角外表面的单层或多层梭形细胞组成，并且可以看到它们从囊袋内明显的单层LEC延伸而来。不同程度地，同时存在散在的色素细胞，推测为葡萄膜来源。膜的细胞成分波形蛋白、转化生长因子-β、碱性成纤维细胞生长因子和平滑肌肌动蛋白染色呈阳性。一种主要由胶原蛋白组成的无定形嗜酸性细胞外基质与这些膜相关。白内障手术后的增殖性前段膜在形态学和免疫组织化学上与后囊膜混浊和过熟期白内障所见的囊膜斑块的细胞和基质成分相似，这两者均由LEC化生和增殖引起。这些LEC衍生的膜与继发性青光眼相关，表明在犬白内障手术后，晶状体囊袋范围外LEC的过度增殖可能导致眼部的病理改变。
