IncI1质粒R64和ColIb-P9的pilL和pilN基因编码负责细菌毛生物合成的外膜脂蛋白。
The pilL and pilN genes of IncI1 plasmids R64 and ColIb-P9 encode outer membrane lipoproteins responsible for thin pilus biogenesis.
作者信息
Sakai D, Komano T
机构信息
Department of Biology, Tokyo Metropolitan University, Minamiohsawa, Hachioji, Tokyo, 192-0397, Japan.
出版信息
Plasmid. 2000 Mar;43(2):149-52. doi: 10.1006/plas.1999.1434.
PMID:10686134
Abstract
The predicted amino acid sequences of the pilL and pilN genes, required for the thin pilus formation of IncI1 plasmids R64 and ColIb-P9, contain N-terminal lipoprotein signal peptide motifs. The pilL and pilN products were labeled with [(3)H]palmitic acid as 38- and 57-kDa proteins, respectively, indicating that they are lipoproteins. Both PilL and PilN were localized to the outer membrane.
摘要
IncI1质粒R64和ColIb - P9形成细菌毛所需的pilL和pilN基因的预测氨基酸序列含有N端脂蛋白信号肽基序。pilL和pilN产物分别被[³H]棕榈酸标记为38 kDa和57 kDa的蛋白质，表明它们是脂蛋白。PilL和PilN都定位于外膜。
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