大鼠纹状体中行为与细胞外多巴胺水平之间的相关性：微透析与快速扫描循环伏安法的比较
Correlation between behavior and extracellular dopamine levels in rat striatum: comparison of microdialysis and fast-scan cyclic voltammetry.
作者信息
Budygin E A, Kilpatrick M R, Gainetdinov R R, Wightman R M
机构信息
Department of Chemistry, Venable Hall, CB 3290, University of North Carolina, Chapel Hill 27599, USA.
出版信息
Neurosci Lett. 2000 Mar 3;281(1):9-12. doi: 10.1016/s0304-3940(00)00813-2.
Recently, fast-scan cyclic voltammetry (FSCV) has been adapted for real-time measurements of evoked dopamine (DA) release and uptake in freely moving rats. Using the advantages of this experimental design in combination with behavioral measures, we examined the effect of GBR 12909 (20 mg/kg, i.p.), a selective DA uptake inhibitor, on striatal extracellular DA dynamics and compared these data to that obtained by microdialysis. These studies established that both techniques report changes in DA that correlate with the kinetics of GBR 12909-induced behavioral effects. However, the time course of changes in evoked DA levels detected by FSCV was more closely linked with the changes in stereotypy than microdialysis measurements.
最近，快速扫描循环伏安法（FSCV）已被应用于自由活动大鼠中诱发多巴胺（DA）释放和摄取的实时测量。利用这种实验设计的优势并结合行为测量，我们研究了选择性DA摄取抑制剂GBR 12909（20 mg/kg，腹腔注射）对纹状体细胞外DA动力学的影响，并将这些数据与通过微透析获得的数据进行比较。这些研究表明，两种技术都报告了与GBR 12909诱导的行为效应动力学相关的DA变化。然而，FSCV检测到的诱发DA水平变化的时间进程比微透析测量更紧密地与刻板行为的变化相关。
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