Lu Y, Peters J L, Michael A C
Department of Chemistry, University of Pittsburgh, Pennsylvania 15260, USA.
J Neurochem. 1998 Feb;70(2):584-93. doi: 10.1046/j.1471-4159.1998.70020584.x.
Carbon fiber microelectrodes either were implanted directly into striatal tissue or were mounted into the outlet of microdialysis probes that were implanted into striatal tissue. This allowed voltammetry and microdialysis to be used under identical in vivo experimental conditions to monitor extracellular dopamine levels during electrical stimulation of the medial forebrain bundle both before and after uptake inhibition with nomifensine. The marked differences between the results obtained with each technique cannot be explained on the basis of their inherent analytical attributes (sensitivity, temporal response, etc.). The results demonstrate that the microdialysis recovery factor for endogenous dopamine increases after uptake inhibition, an observation that stands in contradiction to the existing theory and practice of the microdialysis technique. The observations led to the development of a numerical model that rationalizes the observations reported herein and that allows in vivo voltammetry and in vivo microdialysis results to be interpreted within a single theoretical framework.
碳纤维微电极要么直接植入纹状体组织，要么安装在植入纹状体组织的微透析探针的出口处。这使得伏安法和微透析能够在相同的体内实验条件下使用，以监测在用诺米芬辛抑制摄取前后，内侧前脑束电刺激期间细胞外多巴胺水平。用每种技术获得的结果之间的显著差异不能基于其固有的分析属性（灵敏度、时间响应等）来解释。结果表明，摄取抑制后内源性多巴胺的微透析回收率增加，这一观察结果与微透析技术的现有理论和实践相矛盾。这些观察结果促成了一个数值模型的开发，该模型使本文报道的观察结果合理化，并允许在一个单一的理论框架内解释体内伏安法和体内微透析结果。
