West S D, Turner L G
Global Environmental Chemistry Laboratory-Indianapolis Lab, Dow AgroSciences LLC, 9330 Zionsville Road, Indianapolis, Indiana 46268-1054, USA.
J Agric Food Chem. 2000 Feb;48(2):366-72. doi: 10.1021/jf9903399.
Spinosad is an insect control agent that is derived from a naturally occurring organism and is effective on a wide variety of crops, including citrus crops. A method is described for the determination of spinosad and its metabolites in citrus crops and orange processed commodities. The method determines residues of the active ingredients (spinosyns A and D) and three minor metabolites (spinosyn B, spinosyn K, and N-demethylspinosyn D). For dried orange pulp and orange oil, the method has a limit of quantitation (LOQ) of 0.02 microg/g and a limit of detection (LOD) of 0.006 microg/g. For all other sample matrices (whole fruit, edible fruit, juice, and peel), the method has an LOQ of 0.01 microg/g and an LOD of 0.003 microg/g. The analytes are extracted from the various sample types using appropriate solvents, and the extracts are purified by liquid-liquid partitioning and/or solid-phase extraction. All five analytes are determined simultaneously in the purified extracts by reversed-phase high-performance liquid chromatography with ultraviolet detection at 250 nm.
多杀菌素是一种从天然存在的生物体中提取的昆虫控制剂，对包括柑橘类作物在内的多种作物有效。本文描述了一种测定柑橘类作物及柑橘加工产品中多杀菌素及其代谢物的方法。该方法可测定活性成分（多杀菌素A和D）以及三种次要代谢物（多杀菌素B、多杀菌素K和N-去甲基多杀菌素D）的残留量。对于干橙果肉和橙油，该方法的定量限（LOQ）为0.02微克/克，检测限（LOD）为0.006微克/克。对于所有其他样品基质（整果、可食果实、果汁和果皮），该方法的LOQ为0.01微克/克，LOD为0.003微克/克。使用适当的溶剂从各种样品类型中提取分析物，提取物通过液-液分配和/或固相萃取进行纯化。通过反相高效液相色谱法在250nm波长下进行紫外检测，同时测定纯化提取物中的所有五种分析物。
