West S D, Yeh L T, Turner L G, Schwedler D A, Thomas A D, Duebelbeis D O
Global Environmental Chemistry Laboratory-Indianapolis Laboratory, Dow AgroSciences LLC, 9330 Zionsville Road, Indianapolis, Indiana 46268-1054, USA.
J Agric Food Chem. 2000 Nov;48(11):5131-7. doi: 10.1021/jf000080q.
Spinosad is an insect control agent that is derived from a naturally occurring soil bacterium and is effective on several classes of insects, especially Lepidoptera larvae. Spinosad is registered in many countries for use on a variety of crops, including cotton, corn, soybeans, fruits, and vegetables. Residue methods utilizing high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection have been described for determining spinosad and its metabolites in environmental and food matrices. These residue methods typically involve an extraction with organic solvents, followed by purification using liquid-liquid partitioning and/or solid phase extraction prior to measurement by HPLC-UV. The residue methods determine the active ingredients (spinosyns A and D) and up to three minor metabolites (spinosyn B, spinosyn K, and N-demethylspinosyn D). The methods have validated limits of quantitation ranging from 0.010 to 0.040 microgram/g. This paper briefly reviews the residue methodology for spinosad and metabolites in food and environmental matrices and provides a summary of method validation results for 61 different sample types, including newly published results for 37 additional crop matrices and processed commodities.
多杀菌素是一种昆虫控制剂，它源自一种天然存在的土壤细菌，对几类昆虫有效，尤其是鳞翅目幼虫。多杀菌素在许多国家登记用于多种作物，包括棉花、玉米、大豆、水果和蔬菜。已描述了利用带紫外（UV）检测的高效液相色谱（HPLC）的残留方法，用于测定环境和食品基质中的多杀菌素及其代谢物。这些残留方法通常包括用有机溶剂萃取，然后在通过HPLC-UV测量之前，使用液-液分配和/或固相萃取进行纯化。残留方法可测定活性成分（多杀菌素A和D）以及多达三种次要代谢物（多杀菌素B、多杀菌素K和N-去甲基多杀菌素D）。这些方法的验证定量限范围为0.010至0.040微克/克。本文简要回顾了食品和环境基质中多杀菌素及其代谢物的残留方法，并提供了61种不同样品类型的方法验证结果总结，包括37种额外作物基质和加工商品的新发表结果。
