Hiragun T, Morita E, Shindo H, Tanaka T, Kameyoshi Y, Okabe T, Kanno M, Yamamoto S
Department of Dermatology; Immunology and Parasitology, Hiroshima University School of Medicine, Hiroshima, Japan.
Clin Exp Allergy. 2000 Mar;30(3):433-8. doi: 10.1046/j.1365-2222.2000.00726.x.
An inbred strain of mice, NC, develops dermatitis associated with highly elevated serum IgE and dermal mast cell hyperplasia.
To clarify the mechanisms for the dermal mast cell hyperplasia in NC, we prepared bone marrow-derived mast cells (BMMCs) from three strains of mice, NC/Kuj, C57BL/6 and BALB/c, and compare histamine contents, histamine release abilities, adhesive properties and apoptosis of the BMMCs.
Compared with BMMCs obtained from C57BL/6 and BALB/c, NC/Kuj BMMC possessed higher histamine content and higher adhesive ability to plastic plates, although histamine release from BMMCs was found to be similar in the three strains. The most intriguing finding is the lack of apoptosis in the BMMCs from NC/Kuj upon growth factor deprivation as determined by DNA ladder formation and loss of membrane phospholipid asymmetry.
The altered in vitro properties of mast cells in NC/Kuj partially account for an increase of dermal mast cells, which might be involved in the development of skin lesions in NC.
近交系小鼠NC会发生与血清IgE高度升高及皮肤肥大细胞增生相关的皮炎。
为阐明NC中皮肤肥大细胞增生的机制，我们从NC/Kuj、C57BL/6和BALB/c这三个品系的小鼠制备了骨髓来源的肥大细胞（BMMCs），并比较了BMMCs的组胺含量、组胺释放能力、黏附特性及凋亡情况。
与从C57BL/6和BALB/c获得的BMMCs相比，NC/Kuj BMMC具有更高的组胺含量以及对塑料板更强的黏附能力，不过发现这三个品系的BMMCs组胺释放情况相似。最引人关注的发现是，通过DNA梯状条带形成及膜磷脂不对称性丧失测定，发现NC/Kuj的BMMCs在生长因子剥夺后缺乏凋亡现象。
NC/Kuj中肥大细胞体外特性的改变部分解释了皮肤肥大细胞的增加，这可能与NC皮肤病变的发生有关。
