建立一个无 Kit 突变的肥大细胞系 NCL-2，来源于 NC 小鼠骨髓。
Establishment of a mast cell line, NCL-2, without Kit mutation, derived from NC mouse bone marrow.
机构信息
Department of Dermatology, Integrated Health Sciences, Institute of Biomedical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.
出版信息
FEBS Open Bio. 2014 Apr 2;4:342-6. doi: 10.1016/j.fob.2014.03.011. eCollection 2014.
Immortal mast cell lines, such as RBL-2H3 and HMC-1 cells, are commonly utilized to investigate the function of mast cells. However, they are tumor cells carrying a gain-of-function mutation of Kit. We established an immortal mast cell line without Kit mutation, NCL-2, derived from NC mouse bone marrow. NCL-2 cells could be maintained without additional growth factors and thus could respond to exogenous growth signals. Moreover, NCL-2 cells expressed FcεRI and KIT, and release histamine and LTB4 in response to antigen stimulation. This cell line could be a useful tool to analyze proliferation, differentiation, and function of normal mast cells.
永生肥大细胞系，如 RBL-2H3 和 HMC-1 细胞，常用于研究肥大细胞的功能。然而，它们是携带 Kit 功能获得性突变的肿瘤细胞。我们建立了一种没有 Kit 突变的永生肥大细胞系 NCL-2，源自 NC 小鼠骨髓。NCL-2 细胞可以在没有额外生长因子的情况下维持，因此可以响应外源性生长信号。此外，NCL-2 细胞表达 FcεRI 和 KIT，并在受到抗原刺激时释放组胺和 LTB4。该细胞系可作为分析正常肥大细胞增殖、分化和功能的有用工具。
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