人B细胞系组成性表达并分泌白细胞介素-16。
Human B-cell lines constitutively express and secrete interleukin-16.
作者信息
Sharma V, Sparks J L, Vail J D
机构信息
Laboratory of Cytokine Research, Department of Biology, University of West Florida, Pensacola, FL 32514, USA.
出版信息
Immunology. 2000 Feb;99(2):266-71. doi: 10.1046/j.1365-2567.2000.00959.x.
Abstract
Interleukin-16 (IL-16), produced by activated CD8+ T lymphocytes, is inhibitory to human immunodeficiency virus-1 (HIV-1) replication. In an attempt to determine whether human B cells express and secrete IL-16, a wide panel of B-cell lines derived from patients with acquired immune deficiency syndrome (AIDS)-associated B-cell lymphomas (AABCL) (n = 5) and from non-AABCLs (n = 8) were studied. Using reverse transcription-polymerase chain reaction (RT-PCR) analysis, we were able to observe ubiquitous expression of IL-16 mRNA. Kinetic studies on constitutive mRNA turnover and secretion for IL-16 suggests that the optimum expression is at 24 hr. Interestingly, we report, for the first time, IL-16 secretion by human B-cell lines.
摘要
白细胞介素-16（IL-16）由活化的CD8 + T淋巴细胞产生，可抑制人类免疫缺陷病毒1型（HIV-1）的复制。为了确定人类B细胞是否表达和分泌IL-16，我们研究了一系列广泛的B细胞系，这些细胞系来源于获得性免疫缺陷综合征（AIDS）相关B细胞淋巴瘤（AABCL）患者（n = 5）和非AABCL患者（n = 8）。通过逆转录聚合酶链反应（RT-PCR）分析，我们能够观察到IL-16 mRNA的普遍表达。对IL-16组成型mRNA周转和分泌的动力学研究表明，最佳表达时间为24小时。有趣的是，我们首次报道了人类B细胞系分泌IL-16。
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