Kleinsasser N H, Kastenbauer E R, Weissacher H, Muenzenrieder R K, Harréus U A
Department of Otolaryngology-Head and Neck Surgery, Ludwig-Maximilians-University, Munich, Germany.
Environ Mol Mutagen. 2000;35(1):9-12. doi: 10.1002/(sici)1098-2280(2000)35:1<9::aid-em2>3.0.co;2-1.
Various phthalate compounds are used as softeners and plasticizers in a wide range of plastic materials. There has been a growing concern regarding a possible health hazard to humans. The mucosa of the upper aerodigestive tract is the organ of first contact for the majority of xenobiotics, such as phthalates, entering the body. Still, there is a lack of information concerning possible carcinogenicity of phthalates in the upper aerodigestive tract. This motivated us to investigate their genotoxic effects on human epithelia: human mucosal cells derived from biopsies harvested during surgery of the oropharynx and the inferior nasal turbinate, respectively. The alkaline version of the microgel electrophoresis assay was used to detect single-strand breaks in the DNA following incubation with dibutylphthalate (DBP) and diisobutylphthalate (DiBP). DNA damage was induced by both DBP and DiBP in oropharyngeal and nasal mucosa, though the effect of DiBP was more pronounced than that of DBP. Nasal mucosa proved to be more sensitive than oropharyngeal epithelia. The results demonstrate genotoxic effects of phthalates on human mucosal cells of the upper aerodigestive tract, in contrast to earlier findings in animal models.
各种邻苯二甲酸酯化合物在多种塑料材料中用作软化剂和增塑剂。人们越来越担心其对人类健康可能产生的危害。上呼吸道消化道黏膜是大多数进入人体的外源性物质（如邻苯二甲酸酯）的首要接触器官。然而，关于邻苯二甲酸酯在上呼吸道消化道可能的致癌性，目前仍缺乏相关信息。这促使我们研究它们对人上皮细胞的遗传毒性作用：分别取自口咽和下鼻甲手术活检组织的人黏膜细胞。使用碱性微凝胶电泳试验检测与邻苯二甲酸二丁酯（DBP）和邻苯二甲酸二异丁酯（DiBP）孵育后DNA中的单链断裂。DBP和DiBP均在口咽和鼻黏膜中诱导了DNA损伤，不过DiBP的作用比DBP更明显。鼻黏膜被证明比口咽上皮更敏感。结果表明，与早期在动物模型中的发现相反，邻苯二甲酸酯对人上呼吸道消化道黏膜细胞具有遗传毒性作用。
