大肠杆菌肠杆菌素合酶蛋白EntB/G、EntE和EntF与膜的关联。
Membrane association of the Escherichia coli enterobactin synthase proteins EntB/G, EntE, and EntF.
作者信息
Hantash F M, Earhart C F
机构信息
Section of Molecular Genetics and Microbiology, The University of Texas at Austin, Austin, Texas 78712-1095, USA.
出版信息
J Bacteriol. 2000 Mar;182(6):1768-73. doi: 10.1128/JB.182.6.1768-1773.2000.
Abstract
The cytosolic proteins EntE, EntF, and EntB/G, which are Escherichia coli enzymes necessary for the final stage of enterobactin synthesis, are released by osmotic shock. Here, consistent with the idea that cytoplasmic proteins found in shockates have an affinity for membranes, a small fraction of each was found in membrane preparations. Two procedures demonstrated that the enzymes were enriched in a minor membrane fraction of buoyant density intermediate between that of cytoplasmic and outer membranes, providing indirect support for the notion that these proteins have a role in enterobactin excretion as well as synthesis.
摘要
胞质蛋白EntE、EntF和EntB/G是大肠杆菌合成肠杆菌素最后阶段所必需的酶，它们通过渗透休克释放出来。在这里，与休克液中发现的细胞质蛋白对膜有亲和力这一观点一致，在膜制备物中发现了每种蛋白的一小部分。两种方法表明，这些酶富集在浮力密度介于细胞质膜和外膜之间的一个较小的膜组分中，为这些蛋白在肠杆菌素排泄以及合成中起作用这一观点提供了间接支持。
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