大肠杆菌膜对半甲基化oriC的高亲和力结合是由一个多蛋白系统介导的，该系统包括SeqA和一个新鉴定的因子SeqB。
High-affinity binding of hemimethylated oriC by Escherichia coli membranes is mediated by a multiprotein system that includes SeqA and a newly identified factor, SeqB.
作者信息
Shakibai N, Ishidate K, Reshetnyak E, Gunji S, Kohiyama M, Rothfield L
机构信息
Department of Microbiology, University of Connecticut Health Center, Farmington, CT 06032, USA.
出版信息
Proc Natl Acad Sci U S A. 1998 Sep 15;95(19):11117-21. doi: 10.1073/pnas.95.19.11117.
Abstract
The binding of hemimethylated oriC to Escherichia coli membranes has been implicated in the prevention of premature reinitiation at newly replicated chromosomal origins in a reaction that involves the SeqA protein. We describe the resolution of the membrane-associated oriC-binding activity into two fractions, both of which are required for the high-affinity binding of hemimethylated oriC. The active component in one fraction is identified as SeqA. The active component of the second fraction is a previously undescribed protein factor, SeqB. The reconstituted system reproduced the salient characteristics of the membrane-associated binding activity, suggesting that the membrane-associated oriC-binding machinery of E. coli is likely to be a multiprotein system that includes the SeqA and SeqB proteins.
摘要
半甲基化的oriC与大肠杆菌细胞膜的结合被认为参与了在一个涉及SeqA蛋白的反应中防止新复制的染色体起始位点过早重新起始。我们描述了将与膜相关的oriC结合活性解析为两个组分，这两个组分对于半甲基化oriC的高亲和力结合都是必需的。一个组分中的活性成分被鉴定为SeqA。第二个组分的活性成分是一种先前未描述的蛋白质因子，SeqB。重组系统重现了与膜相关的结合活性的显著特征，这表明大肠杆菌的与膜相关的oriC结合机制可能是一个包括SeqA和SeqB蛋白的多蛋白系统。
相似文献
1
High-affinity binding of hemimethylated oriC by Escherichia coli membranes is mediated by a multiprotein system that includes SeqA and a newly identified factor, SeqB.大肠杆菌膜对半甲基化oriC的高亲和力结合是由一个多蛋白系统介导的，该系统包括SeqA和一个新鉴定的因子SeqB。
Proc Natl Acad Sci U S A. 1998 Sep 15;95(19):11117-21. doi: 10.1073/pnas.95.19.11117.
2
Competition between the replication initiator DnaA and the sequestration factor SeqA for binding to the hemimethylated chromosomal origin of E. coli in vitro.复制起始蛋白DnaA与隔离因子SeqA在体外结合大肠杆菌半甲基化染色体起源位点的竞争。
Genes Cells. 2000 Nov;5(11):873-884. doi: 10.1046/j.1365-2443.2000.00380.x.
3
Interaction of SeqA and Dam methylase on the hemimethylated origin of Escherichia coli chromosomal DNA replication.SeqA与Dam甲基化酶在大肠杆菌染色体DNA复制的半甲基化起始位点上的相互作用。
J Biol Chem. 1999 Apr 23;274(17):11463-8. doi: 10.1074/jbc.274.17.11463.
4
The Escherichia coli SeqA protein binds specifically to two sites in fully and hemimethylated oriC and has the capacity to inhibit DNA replication and affect chromosome topology.大肠杆菌SeqA蛋白能特异性结合完全甲基化和半甲基化oriC中的两个位点，并具有抑制DNA复制和影响染色体拓扑结构的能力。
Biochimie. 2001 Jan;83(1):49-51. doi: 10.1016/s0300-9084(00)01207-4.
5
E. coli SeqA protein binds oriC in two different methyl-modulated reactions appropriate to its roles in DNA replication initiation and origin sequestration.大肠杆菌SeqA蛋白通过两种不同的甲基调节反应与oriC结合，这两种反应与其在DNA复制起始和复制起点隔离中的作用相适应。
Cell. 1995 Sep 22;82(6):927-36. doi: 10.1016/0092-8674(95)90272-4.
6
The Escherichia coli SeqA protein binds specifically and co-operatively to two sites in hemimethylated and fully methylated oriC.大肠杆菌SeqA蛋白能特异性地与半甲基化和完全甲基化的oriC中的两个位点协同结合。
Mol Microbiol. 2000 Jun;36(6):1319-26. doi: 10.1046/j.1365-2958.2000.01943.x.
7
Bidirectional migration of SeqA-bound hemimethylated DNA clusters and pairing of oriC copies in Escherichia coli.大肠杆菌中SeqA结合的半甲基化DNA簇的双向迁移及oriC拷贝的配对
Genes Cells. 2000 May;5(5):327-41. doi: 10.1046/j.1365-2443.2000.00334.x.
8
SeqA, the Escherichia coli origin sequestration protein, can regulate the replication of the pBR322 plasmid. SeqA，一种大肠杆菌起源的隔离蛋白，能够调控 pBR322 质粒的复制。
Plasmid. 2011 Jan;65(1):15-9. doi: 10.1016/j.plasmid.2010.09.003. Epub 2010 Sep 25.
9
Replication cycle dependent association of SeqA to the outer membrane fraction of E. coli.SeqA与大肠杆菌外膜组分的复制周期依赖性关联。
Biochimie. 1999 Aug-Sep;81(8-9):841-6. doi: 10.1016/s0300-9084(99)00212-6.
10
Escherichia coli SeqA protein affects DNA topology and inhibits open complex formation at oriC.大肠杆菌SeqA蛋白影响DNA拓扑结构并抑制oriC处开放复合物的形成。
EMBO J. 1999 Sep 1;18(17):4882-8. doi: 10.1093/emboj/18.17.4882.
引用本文的文献
1
DNA Transactions in Bacteria and Membranes: A Place for the Hfq Protein?细菌与膜中的DNA交易：Hfq蛋白的作用？
Membranes (Basel). 2025 Apr 1;15(4):103. doi: 10.3390/membranes15040103.
2
60 Years of Studies into the Initiation of Chromosome Replication in Bacteria.60年细菌染色体复制起始研究
Biomolecules. 2025 Feb 1;15(2):203. doi: 10.3390/biom15020203.
3
Hypothesis: bacteria live on the edge of phase transitions with a cell cycle regulated by a water-clock.假说：细菌生活在相变的边缘，其细胞周期受水钟调控。
Theory Biosci. 2024 Nov;143(4):253-277. doi: 10.1007/s12064-024-00427-2. Epub 2024 Nov 6.
4
Open Questions about the Roles of DnaA, Related Proteins, and Hyperstructure Dynamics in the Cell Cycle.关于DnaA、相关蛋白以及超结构动力学在细胞周期中作用的开放性问题。
Life (Basel). 2023 Sep 10;13(9):1890. doi: 10.3390/life13091890.
5
Epigenetic memory via concordant DNA methylation is inversely correlated to developmental potential of mammalian cells.通过一致的DNA甲基化形成的表观遗传记忆与哺乳动物细胞的发育潜能呈负相关。
PLoS Genet. 2017 Nov 6;13(11):e1007060. doi: 10.1371/journal.pgen.1007060. eCollection 2017 Nov.
6
The membrane: transertion as an organizing principle in membrane heterogeneity.膜：作为膜异质性组织原则的转运
Front Microbiol. 2015 Jun 12;6:572. doi: 10.3389/fmicb.2015.00572. eCollection 2015.
7
Chromosomal replication initiation machinery of low-G+C-content Firmicutes.低 G+C 含量厚壁菌门的染色体复制起始机制。
J Bacteriol. 2012 Oct;194(19):5162-70. doi: 10.1128/JB.00865-12. Epub 2012 Jul 13.
8
Isolation of a new hemimethylated DNA binding protein which regulates dnaA gene expression.一种调节dnaA基因表达的新型半甲基化DNA结合蛋白的分离。
J Bacteriol. 2003 May;185(9):2967-71. doi: 10.1128/JB.185.9.2967-2971.2003.
9
A case for sliding SeqA tracts at anchored replication forks during Escherichia coli chromosome replication and segregation.关于大肠杆菌染色体复制和分离过程中，在固定的复制叉处滑动SeqA序列片段的一个实例。
EMBO J. 2000 Nov 15;19(22):6249-58. doi: 10.1093/emboj/19.22.6249.
10
The eclipse period of Escherichia coli.大肠杆菌的隐蔽期。
EMBO J. 2000 Nov 15;19(22):6240-8. doi: 10.1093/emboj/19.22.6240.
本文引用的文献
1
Transmembrane topology of the Rieske Fe/S protein of the cytochrome b6/f complex from spinach chloroplasts.菠菜叶绿体细胞色素b6/f复合体中铁硫蛋白的跨膜拓扑结构
FEBS Lett. 1997 May 19;408(2):206-10. doi: 10.1016/s0014-5793(97)00427-4.
2
Coordinating DNA replication initiation with cell growth: differential roles for DnaA and SeqA proteins.协调DNA复制起始与细胞生长：DnaA蛋白和SeqA蛋白的不同作用
Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12206-11. doi: 10.1073/pnas.93.22.12206.
3
The initiation cascade for chromosome replication in wild-type and Dam methyltransferase deficient Escherichia coli cells.野生型和Dam甲基转移酶缺陷型大肠杆菌细胞中染色体复制的起始级联反应。
EMBO J. 1994 Apr 15;13(8):1856-62. doi: 10.1002/j.1460-2075.1994.tb06454.x.
4
The initiator protein DnaA: evolution, properties and function.起始蛋白DnaA：进化、特性与功能
Biochim Biophys Acta. 1994 Mar 1;1217(2):111-30. doi: 10.1016/0167-4781(94)90025-6.
5
SeqA: a negative modulator of replication initiation in E. coli.SeqA：大肠杆菌中复制起始的负调控因子。
Cell. 1994 May 6;77(3):413-26. doi: 10.1016/0092-8674(94)90156-2.
6
Parental strand recognition of the DNA replication origin by the outer membrane in Escherichia coli.大肠杆菌外膜对DNA复制起点的亲本链识别
EMBO J. 1994 Oct 3;13(19):4695-703. doi: 10.1002/j.1460-2075.1994.tb06793.x.
7
A protein that binds to the P1 origin core and the oriC 13mer region in a methylation-specific fashion is the product of the host seqA gene.一种以甲基化特异性方式结合P1起始核心和oriC 13聚体区域的蛋白质是宿主seqA基因的产物。
EMBO J. 1995 Aug 15;14(16):4083-9. doi: 10.1002/j.1460-2075.1995.tb00080.x.
8
E. coli SeqA protein binds oriC in two different methyl-modulated reactions appropriate to its roles in DNA replication initiation and origin sequestration.大肠杆菌SeqA蛋白通过两种不同的甲基调节反应与oriC结合，这两种反应与其在DNA复制起始和复制起点隔离中的作用相适应。
Cell. 1995 Sep 22;82(6):927-36. doi: 10.1016/0092-8674(95)90272-4.
9
Regulation of chromosomal replication in E. coli: sequestration and beyond.大肠杆菌中染色体复制的调控：隔离及其他方面。
Cell. 1995 Sep 22;82(6):877-80. doi: 10.1016/0092-8674(95)90020-9.
10
Purification of bacterial membrane proteins. The use of guanidinium thiocyanate and urea.细菌膜蛋白的纯化。硫氰酸胍和尿素的应用。
J Membr Biol. 1972;10(2):137-52. doi: 10.1007/BF01867850.
Zotero 插件