长期摄入乙醇可提高大鼠肝脏线粒体中氧化磷酸化的效率。

Chronic ethanol ingestion increases efficiency of oxidative phosphorylation in rat liver mitochondria.

作者信息

Piquet M A, Nogueira V, Devin A, Sibille B, Filippi C, Fontaine E, Roulet M, Rigoulet M, Leverve X M

机构信息

Laboratoire de Bioénergétique Fondamentale et Appliquée, UJF, P.O. Box 53X, 38041, Grenoble, France.

出版信息

FEBS Lett. 2000 Feb 25;468(2-3):239-42. doi: 10.1016/s0014-5793(00)01225-4.

DOI:10.1016/s0014-5793(00)01225-4

PMID:10692594

Abstract

The efficiency of oxidative phosphorylation was compared between rats chronically fed with ethanol and controls. (i) Results showed that the liver mitochondria state 4 respiratory rate was strongly inhibited, while the corresponding proton-motive force was not affected; (ii) the cytochrome oxidase content and activity were decreased and (iii) the oxidative-phosphorylation yield was increased in the ethanol exposed group. Furthermore, oxidative phosphorylation at coupling site II was not affected by ethanol. Cytochrome oxidase inhibition by sodium-azide mimicked the effects of ethanol intoxication in control mitochondria. This indicates that the decrease in cytochrome oxidase activity induced by ethanol intoxication directly increases the efficiency of oxidative phosphorylation.

摘要

对长期喂食乙醇的大鼠和对照组大鼠的氧化磷酸化效率进行了比较。(i) 结果显示，肝脏线粒体状态4呼吸速率受到强烈抑制，而相应的质子动力未受影响；(ii) 细胞色素氧化酶含量和活性降低；(iii) 乙醇暴露组的氧化磷酸化产量增加。此外，偶联位点II处的氧化磷酸化不受乙醇影响。叠氮化钠对细胞色素氧化酶的抑制作用模拟了乙醇中毒对对照线粒体的影响。这表明乙醇中毒诱导的细胞色素氧化酶活性降低直接提高了氧化磷酸化的效率。