Möller P, Andersson P O, Andersson A C, Wadenvik H
Department of Internal Medicine, Section for Haematology, Sahlgrenska University Hospital, Göteborg University, Göteborg, Sweden.
Acta Haematol. 2000;102(3):131-4. doi: 10.1159/000040986.
Studies performed in rabbit and mouse models and in a limited number of human subjects, show that transfused platelets bind thrombopoietin (TPO) and decrease its concentration in the circulation. The aim of the present study was to further examine this relationship. The material comprised 12 patients receiving a total of 21 transfusions, as part of the routine clinical treatment. Blood samples were collected from the patients immediately before and 30 min after completion of the platelet transfusion, and the corrected platelet count increment (CCI) was calculated. A commercially available ELISA kit was used to determine plasma TPO concentrations. Statistically significant reductions in median TPO concentration were observed in response to the platelet transfusions. Patients who were refractory to platelet transfusions showed the slightest decrease in TPO concentration. As for the linear regression between change in TPO level and CCI, only borderline significance was observed. Thus, our findings support the concept that platelets can remove TPO from the circulation.
在兔和小鼠模型以及有限数量的人类受试者中进行的研究表明，输注的血小板会结合血小板生成素（TPO）并降低其在循环中的浓度。本研究的目的是进一步研究这种关系。材料包括12名患者，作为常规临床治疗的一部分，共接受了21次输血。在血小板输注完成前和完成后30分钟立即从患者采集血样，并计算校正血小板计数增加值（CCI）。使用市售ELISA试剂盒测定血浆TPO浓度。观察到血小板输注后中位数TPO浓度有统计学意义的降低。对血小板输注难治的患者TPO浓度下降最少。至于TPO水平变化与CCI之间的线性回归，仅观察到临界显著性。因此，我们的研究结果支持血小板可从循环中清除TPO这一概念。
