Aly N M, el-Gendy K S
Pesticide Central Lab., Agriculture Research Center, Alexandria, Egypt.
J Environ Sci Health B. 2000 Jan;35(1):77-86. doi: 10.1080/03601230009373255.
The functional status of the immune system of female mice exposed to a single oral dose of dimethoate (16 mg/kg) was evaluated by assessing cell mediated and humoral immune responses, in addition to the effect of dimethoate on spleen and body weights after different time intervals. The data showed that dimethoate caused a time-depended decrease in spleen weights in the absence of a change in body weights. The immunologic effect of dimethoate to female mice produced a dose-dependent decrease in the number of the rosette forming cells (total and active erythrocyte rosette). The ability of splenocytes to proliferation in response to mitogens; phytohemagglutinin (PHA) for T cell and lipopolysaccharide (LPS) for B cell were significantly decreased at the different times. As compared to control, a significant decrease in serum total immunoglobulins (Ig) and IgM was found, while IgG was non-significant deceased. Results of this study also revealed that dimethoate caused a significant decrease in the number of plaque forming cell (PFC/10(6) splenocytes) in a time dependent manner.
通过评估细胞介导免疫和体液免疫反应，以及在不同时间间隔后乐果对脾脏重量和体重的影响，来评价单次口服乐果（16毫克/千克）的雌性小鼠免疫系统的功能状态。数据表明，在体重无变化的情况下，乐果导致脾脏重量呈时间依赖性下降。乐果对雌性小鼠的免疫作用使玫瑰花结形成细胞（总红细胞玫瑰花结和活性红细胞玫瑰花结）数量呈剂量依赖性减少。在不同时间，脾细胞对有丝分裂原（T细胞用植物血凝素，B细胞用脂多糖）的增殖能力显著降低。与对照组相比，血清总免疫球蛋白（Ig）和IgM显著降低，而IgG虽有下降但不显著。本研究结果还显示，乐果使空斑形成细胞数量（PFC/10⁶脾细胞）呈时间依赖性显著减少。
