Biteau N, Bringaud F, Gibson W, Truc P, Baltz T
Laboratoire de Parasitologie Moléculaire, Université Victor Segalen de Bordeaux II, UPRESA-5016 CNRS, France.
Mol Biochem Parasitol. 2000 Feb 5;105(2):185-201.
Genetic variation of microsatellite loci is a widely used method for linkage analysis, individual identification or inter-population studies. Here we analyse a repeated DNA coding sequence and eleven new microsatellites identified within the Trypanosoma (Trypanozoon) brucei genome. Ninety-seven isolates belonging to the five species and subspecies Trypanosoma evansi, T. equiperdum, T. brucei brucei, T. b. rhodesiense and T. b. gambiense were compared regarding the genetic patterns of these markers. The results reveal a great heterogeneity of the genotypes related to the repeated coding sequence and five microsatellites, some of which show a high degree of polymorphism. This allows us to define group-specific genotypes or alleles; in particular, we show that one specific pattern clearly segregates the human pathogen T. b. gambiense group I.
微卫星位点的遗传变异是一种广泛用于连锁分析、个体识别或群体间研究的方法。在此，我们分析了布氏锥虫（锥虫亚属）基因组内一个重复的DNA编码序列和11个新的微卫星。比较了属于伊氏锥虫、马媾疫锥虫、布氏锥虫、罗德西亚布氏锥虫和冈比亚布氏锥虫这五个物种和亚种的97个分离株的这些标记的遗传模式。结果揭示了与重复编码序列和五个微卫星相关的基因型的高度异质性，其中一些显示出高度多态性。这使我们能够定义群体特异性基因型或等位基因；特别是，我们表明一种特定模式能清楚地将人类病原体冈比亚布氏锥虫第一组区分开来。
