Wen Yan-Zi, Lun Zhao-Rong, Zhu Xing-Quan, Hide Geoff, Lai De-Hua
Center for Parasitic Organisms, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.
Center for Parasitic Organisms, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China; State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, PR China; Ecosystems & Environment Centre, School of Environment and Life Sciences, University of Salford, Salford M5 4WT, UK; Biomedical Research Centre, School of Environment and Life Sciences, University of Salford, Salford M5 4WT, UK.
Infect Genet Evol. 2016 Jul;41:56-62. doi: 10.1016/j.meegid.2016.03.022. Epub 2016 Mar 23.
The subgenus Trypanozoon includes three species Trypanosoma brucei, Trypanosoma evansi and Trypanosoma equiperdum, which are morphologically identical and indistinguishable even using some molecular methods. In this study, PCR-based single strand conformation polymorphism (PCR-SSCP) was used to analyze the ribosomal DNA of the Trypanozoon species. Data indicate different patterns of ITS2 fragments between T. brucei, T. evansi and T. equiperdum by SSCP. Furthermore, analysis of total ITS sequences within these three members of the subgenus Trypanozoon showed a high degree of homology using phylogenetic analysis but were polyphyletic in haplotype networks. These data provide novel nuclear evidence to further support the notion that T. evansi and T. equiperdum should be subspecies or even strains of T. brucei.
布氏锥虫、伊氏锥虫和马媾疫锥虫，它们在形态上相同，即使使用一些分子方法也无法区分。在本研究中，基于聚合酶链反应的单链构象多态性（PCR-SSCP）被用于分析锥虫亚属物种的核糖体DNA。数据表明，通过SSCP分析，布氏锥虫、伊氏锥虫和马媾疫锥虫的ITS2片段呈现出不同的模式。此外，对锥虫亚属这三个成员的总ITS序列进行系统发育分析显示出高度的同源性，但在单倍型网络中是多系的。这些数据提供了新的核证据，进一步支持伊氏锥虫和马媾疫锥虫应是布氏锥虫的亚种甚至菌株的观点。
