Lee K J, Dietrich P, Jessell T M
Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Centre for Neurobiology and Behavior, Columbia University, New York, New York 10032, USA.
Nature. 2000 Feb 17;403(6771):734-40. doi: 10.1038/35001507.
During neural development in vertebrates, a spatially ordered array of neurons is generated in response to inductive signals derived from localized organizing centres. One organizing centre that has been proposed to have a role in the control of neural patterning is the roof plate. To define the contribution of signals derived from the roof plate to the specification of neuronal cell types in the dorsal neural tube, we devised a genetic strategy to ablate the roof plate selectively in mouse embryos. Embryos without a roof plate lack all the interneuron subtypes that are normally generated in the dorsal third of the neural tube. Using a genetically based lineage analysis and in vitro assays, we show that the loss of these neurons results from the elimination of non-autonomous signals provided by the roof plate. These results reveal that the roof plate is essential for specifying multiple classes of neurons in the mammalian central nervous system.
在脊椎动物的神经发育过程中，响应来自局部组织中心的诱导信号，会产生空间有序排列的神经元。有人提出，一个在神经模式控制中起作用的组织中心是顶板。为了确定源自顶板的信号对背侧神经管中神经元细胞类型特化的贡献，我们设计了一种基因策略，以在小鼠胚胎中选择性地消融顶板。没有顶板的胚胎缺乏通常在神经管背侧三分之一处产生的所有中间神经元亚型。使用基于基因的谱系分析和体外试验，我们表明这些神经元的缺失是由于顶板提供的非自主信号的消除。这些结果表明，顶板对于在哺乳动物中枢神经系统中指定多类神经元至关重要。
