Newman S A, Pautou M P, Kieny M
Department of Anatomy, New York Medical College, Valhalla, New York 10595, USA.
Dev Biol. 1981 Jun;84(2):440-8. doi: 10.1016/0012-1606(81)90413-9.
Using chimeras consisting of chick embryos that had received substitution grafts of quail somites, we have determined the distalmost extension of the myogenic primordia in the outgrowing wing bud at 5 days of incubation. At Hamburger-Hamilton stage 25 the most distal premuscle cell is consistently 300 mum or more from the apex of the wing mesoblast. The stage 25 wing tip resembles very early whole limb buds in not having proceeded beyond the mesenchymal state or having expressed markers of terminal differentiation. However, unlike early whole limb buds it is free of a myogenic subpopulation. We therefore propose that the stage 25 wing tip is the appropriate system for in vitro and molecular studies of cartilage differentiation.
我们利用由接受了鹌鹑体节替代移植的鸡胚组成的嵌合体，确定了孵化5天时正在生长的翅芽中肌源性原基的最远端延伸。在汉伯格-汉密尔顿第25阶段，最远端的前肌细胞始终距离翅中胚层顶端300微米或更远。第25阶段的翅尖类似于非常早期的整个肢体芽，尚未超越间充质状态或表达终末分化标记。然而，与早期的整个肢体芽不同，它没有肌源性亚群。因此，我们认为第25阶段的翅尖是用于软骨分化体外和分子研究的合适系统。
