体积加权平均核体积。这个新的预后指标在不同机构中具有可比性吗？

Volume-weighted mean nuclear volume. Is this new prognosticator comparable in different institutions?

作者信息

Fujikawa K, Aoyama T, Itoh T, Nishio Y, Miyakawa M, Sasaki M

机构信息

Department of Urology, Kobe City General Hospital, Japan.

出版信息

Anal Quant Cytol Histol. 2000 Feb;22(1):55-62.

PMID:10696461

Abstract

OBJECTIVE

To determine whether volume-weighted mean nuclear volume (MNV) obtained at one institution is comparable to that from other institutions.

STUDY DESIGN

MNV calculated from histologic slides obtained at three hospitals--Shizuoka Prefectural Hospital (SPH), Shimada Municipal Hospital (SMH) and Shizuoka City Hospital (SCH)--were compared. Between December 1994 and June 1996, transurethral resection of bladder tumor or transurethral resection of the prostate was performed on 37 patients at SPH and 50 patients at SMH; histologic specimens from 40 cases of bladder tumors, 63 cases of normal bladder mucosa, 28 cases of benign prostatic hyperplasia and 1 case of prostate cancer were obtained. A portion of each specimen obtained at SPH or SMH was carried to SCH, and histologic slides were made at SCH using it. Using the remaining position of each specimen, histologic slides were then prepared at each hospital. Estimates of MNV were made from all histologic slides from each hospital, and the differences in MNV between the hospitals were analyzed. In addition, intraobserver and interobserver reproducibility were analyzed using 50 specimens obtained between December 1994 and August 1995.

RESULTS

On linear regression analysis, comparison of MNVs calculated from the histologic slides from SPH and SCH and those calculated from SMH and SCH revealed high correlation coefficients (R = .966 and .966, respectively), and the slope of the regression line did not differ significantly from unity. The paired t test also disclosed no significant difference between MNVs calculated at the two hospitals. Furthermore, the correlation coefficients for intraobserver and interobserver reproducibility of MNV estimates were also high (R = .918 and .949, respectively).

CONCLUSION

The results of this study indicate that estimates of MNV are comparable in multiple institutions, and we recommend that they be used to support subjective histologic grading.

摘要

目的

确定在一个机构获得的体积加权平均核体积（MNV）是否与其他机构的可比。

研究设计

比较了从静冈县立医院（SPH）、岛田市立医院（SMH）和静冈市医院（SCH）三家医院获取的组织学切片计算得出的MNV。1994年12月至1996年6月期间，SPH对37例患者进行了膀胱肿瘤经尿道切除术或前列腺经尿道切除术，SMH对50例患者进行了同样的手术；获取了40例膀胱肿瘤、63例正常膀胱黏膜、28例良性前列腺增生和1例前列腺癌的组织学标本。在SPH或SMH获取的每个标本的一部分被送到SCH，并在SCH用其制作组织学切片。然后使用每个标本的剩余部分在各医院制作组织学切片。从各医院的所有组织学切片中估计MNV，并分析各医院之间MNV的差异。此外，使用1994年12月至1995年8月期间获取的50个标本分析了观察者内和观察者间的可重复性。

结果

线性回归分析显示，比较SPH和SCH的组织学切片计算得出的MNV与SMH和SCH计算得出的MNV，相关系数较高（分别为R = 0.966和0.966），回归线的斜率与1无显著差异。配对t检验也显示两家医院计算得出的MNV之间无显著差异。此外，MNV估计的观察者内和观察者间可重复性的相关系数也很高（分别为R = 0.918和0.949）。