Niven D F, Ekins A, al-Samaurai A A
Department of Natural Resource Sciences, McGill University, Ste. Anne de Bellevue, QC, Canada.
Can J Microbiol. 1999 Dec;45(12):1027-32. doi: 10.1139/w99-114.
A complex medium supported good growth of the type strain of Streptococcus suis irrespective of the presence or absence of a high concentration (1 microM) of the iron chelating agent, ethylenediamine di-o-hydroxyphenylacetic acid. Good growth was also obtained using a complex medium that had been treated with Chelex-100 to reduce the iron content, but only if this medium was supplemented with manganese; supplementation with iron had little effect. Collectively, these results indicate that S. suis requires manganese, but not iron, for growth. Polyacrylamide gel electrophoresis of cell extracts followed by activity staining revealed the presence of a single manganese-cofactored superoxide dismutase; activity staining and enzyme assays revealed that manganese availability during growth affected the activity of the superoxide dismutase in cell extracts. The results are discussed with respect to the capacity of S. suis to avoid damage by reactive oxygen species.
复杂培养基能支持猪链球菌模式菌株良好生长，无论是否存在高浓度（1微摩尔）的铁螯合剂乙二胺二邻羟基苯乙酸。使用经Chelex - 100处理以降低铁含量的复杂培养基也能实现良好生长，但前提是该培养基补充了锰；补充铁的效果甚微。总体而言，这些结果表明猪链球菌生长需要锰而非铁。对细胞提取物进行聚丙烯酰胺凝胶电泳，随后进行活性染色，结果显示存在一种单一的锰辅助超氧化物歧化酶；活性染色和酶活性测定表明，生长过程中锰的可利用性会影响细胞提取物中超氧化物歧化酶的活性。针对猪链球菌避免活性氧损伤的能力对结果进行了讨论。
