从白线天蛾（Hyles lineata）中分离和鉴定与促肾上腺皮质激素释放因子相关的利尿激素。
Isolation and characterization of CRF-related diuretic hormones from the whitelined sphinx moth Hyles lineata.
作者信息
Furuya K, Harper M A, Schegg K M, Schooley D A
机构信息
Department of Biochemistry, University of Nevada, Reno 89557-0014, USA.
出版信息
Insect Biochem Mol Biol. 2000 Feb;30(2):127-33. doi: 10.1016/s0965-1748(99)00106-x.
We have isolated and characterized two diuretic hormones (DH), Hylli-DH41 and Hylli-DH30, from extracts of whole heads of the lepidopteran Hyles lineata. We monitored the isolation by measuring the ability of fractions to affect levels of cyclic AMP production by Malpighian tubules of Manduca sexta maintained in vitro. These DH are related to a family of vertebrate neuropeptides which includes sauvagine, corticotropin-releasing factor (CRF), and urotensin I. Both Hylli-DH41 (RMPSLSIDLPMSVLRQKLSLE KERKVQALRAAANRNFLNDI-NH2) and Hylli-DH30 (SFSVNPAVEILQHRYMEKVAQNNRNFLNRV-NH2) show extremely high similarity with two DH from the tobacco hornworm M. sexta. This is not surprising because both H. lineata and M. sexta are sphingid moths. The discovery of these DH provides a third example of two CRF-related DH occurring in one insect species.
我们从鳞翅目昆虫烟草天蛾（Hyles lineata）的全头部提取物中分离并鉴定了两种利尿激素（DH），即Hylli-DH41和Hylli-DH30。我们通过测量各组分影响体外培养的烟草天蛾（Manduca sexta）马氏管中环状AMP生成水平的能力来监测分离过程。这些利尿激素与一类脊椎动物神经肽家族有关，该家族包括蛙皮素、促肾上腺皮质激素释放因子（CRF）和尾加压素I。Hylli-DH41（RMPSLSIDLPMSVLRQKLSLE KERKVQALRAAANRNFLNDI-NH2）和Hylli-DH30（SFSVNPAVEILQHRYMEKVAQNNRNFLNRV-NH2）与烟草天蛾的两种利尿激素都显示出极高的相似性。这并不奇怪，因为烟草天蛾和烟草天蛾都是天蛾科蛾类。这些利尿激素的发现为在一个昆虫物种中出现两种与CRF相关的利尿激素提供了第三个例子。
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