Lokshin A, Zhang H, Mayotte J, Lokshin M, Levitt M L
Lab 320 Magee Women's Research Institute, Pittsburgh, PA 15213, USA.
Anticancer Res. 1999 Nov-Dec;19(6B):5251-4.
Retinoids represent a potentially useful class of drugs in the chemoprevention and treatment of cancer, due to their ability to regulate cell proliferation and differentiation. However, there is controversy in the literature about the effects of all-trans retinoic acid (ATRA) in non- small cell lung cancer (NSCLC). In this study we examined the effects of ATRA on apoptotic death in NSCLC.
Cell proliferation was determined by thymidine incorporation in cultured NSCLC cells. DNA fragmentation was measured in NSCLC cell lines as a marker of apoptosis. The expression of keratinocyte transglutaminase and cytokeratin 10 were measured as markers of squamous differentiation.
ATRA inhibited cell proliferation, and induced markers of both apoptosis and squamous differentiation after 24-48 hrs of treatment.
These results indicate the possibility of the early growth-inhibitory and apoptotic effects of ATRA in NSCLC which may result in selection of ATRA-resistant cells.
由于类视黄醇具有调节细胞增殖和分化的能力，它们在癌症的化学预防和治疗中代表了一类潜在有用的药物。然而，关于全反式维甲酸（ATRA）在非小细胞肺癌（NSCLC）中的作用，文献中存在争议。在本研究中，我们研究了ATRA对NSCLC细胞凋亡死亡的影响。
通过在培养的NSCLC细胞中掺入胸苷来测定细胞增殖。在NSCLC细胞系中测量DNA片段化作为细胞凋亡的标志物。测量角质形成细胞转谷氨酰胺酶和细胞角蛋白10的表达作为鳞状分化的标志物。
ATRA抑制细胞增殖，并在处理24 - 48小时后诱导细胞凋亡和鳞状分化的标志物。
这些结果表明ATRA在NSCLC中具有早期生长抑制和凋亡作用的可能性，这可能导致选择ATRA耐药细胞。
