Kawamura K, Ishiwata K, Futatsubashi M, Ishii S I, Ouchi Y, Homma Y, Senda M
Positron Medical Center, Tokyo Metropolitan Institute of Gerontology, Japan.
Appl Radiat Isot. 2000 Feb;52(2):225-8. doi: 10.1016/s0969-8043(99)00152-9.
HPLC separation of either [11C]beta-CFT or [11C]beta-CIT from the N-desmethyl precursor greatly depended on the ODS column used and the pH of the phosphate buffer in the mobile phase. The separation was accomplished on the semipreparative reversed phase ODS column without end-capping treatment over a pH ranging from 6.4 to 9.2, but failed on the end-capped ODS column. This suggests that the presence of residual silanol groups on the ODS is an important factor for the separation.
从N-去甲基前体中通过高效液相色谱法（HPLC）分离[11C]β-CFT或[11C]β-CIT在很大程度上取决于所用的十八烷基硅烷键合硅胶（ODS）柱以及流动相中磷酸盐缓冲液的pH值。在未经封端处理的半制备反相ODS柱上，在pH值为6.4至9.2的范围内可实现分离，但在封端的ODS柱上则失败。这表明ODS上残留硅醇基的存在是分离的一个重要因素。
