Dolo A, Modiano D, Doumbo O, Bosman A, Sidibé T, Keita M M, Naitza S, Robson K J, Crisanti A
Départment d'Epidémiologie des Affections Parasitaires, Ecole Nationale de Médecine, de Pharmacie et d'Odonto-Stomatologie, Bamako, Mali.
Parassitologia. 1999 Sep;41(1-3):425-8.
We have investigated whether naturally induced immunity to Plasmodium falciparum thrombospondin related adhesive protein contributes to protection against malaria in humans. We have carried out a case control study in children living in an endemic region of West Africa to reveal associations between PfTRAP seroprevalence and the risk of cerebral malaria. Sera collected from the case and control groups were analysed by ELISA to compare their serum reactivity against PfTRAP, the circumsporozoite protein and the merozoite surface protein 1. Children with uncomplicated malaria had a significantly higher PfTRAP seroprevalence when compared to children with cerebral malaria. The risk of developing cerebral malaria appeared to depend on the reciprocal relationship between sporozoite inoculation rates and humoral immunity against PfTRAP. Our results suggest that naturally induced humoral immunity against PfTRAP contributes to the development of protection against severe malaria. Experimentally induced immunity against TRAP in different rodent models has consistently proven to elicit a high degree of protection against malaria. This together with the functional properties of TRAP and data describing CD4 and CD8 epitopes for PfTRAP indicate that this molecule could increase the protective efficiency of available sporozoite malaria vaccines.
我们研究了自然诱导产生的针对恶性疟原虫血小板反应蛋白相关黏附蛋白（PfTRAP）的免疫力是否有助于人类预防疟疾。我们在西非疟疾流行地区的儿童中开展了一项病例对照研究，以揭示PfTRAP血清阳性率与脑型疟疾风险之间的关联。通过酶联免疫吸附测定（ELISA）分析病例组和对照组采集的血清，比较它们对PfTRAP、环子孢子蛋白和裂殖子表面蛋白1的血清反应性。与患脑型疟疾的儿童相比，患非重症疟疾的儿童PfTRAP血清阳性率显著更高。患脑型疟疾的风险似乎取决于子孢子接种率与针对PfTRAP的体液免疫之间的相互关系。我们的结果表明，自然诱导产生的针对PfTRAP的体液免疫有助于形成针对重症疟疾的保护作用。在不同啮齿动物模型中通过实验诱导产生的针对TRAP的免疫力一直被证明能引发高度的抗疟保护作用。这与TRAP的功能特性以及描述PfTRAP的CD4和CD8表位的数据表明，该分子可提高现有子孢子疟疾疫苗的保护效率。
