Seki N, Hattori A, Sugano S, Suzuki Y, Nakagawara A, Ohhira M, Muramatsu M, Hori T, Saito T
Genome Research Group, National Institute of Radiological Sciences, Chiba, Japan.
J Hum Genet. 1998;43(4):272-4. doi: 10.1007/s100380050088.
We identified a novel gene encoding a RING finger (C3HC4-type zinc finger) protein from a human neuroblastoma full-length enriched cDNA library. This cDNA clone consists of 1919 nucleotides with an open reading frame of a 485-amino acid protein. From reverse transcription (RT)-polymerase chain reaction (PCR) analysis, the messenger RNA was ubiquitously expressed in various human adult tissues. The chromosomal location of the gene was determined on the chromosome 6p21.3 region by PCR-based analyses with both a human/rodent monochromosomal hybrid cell panel and a radiation hybrid mapping panel.
我们从人神经母细胞瘤全长富集cDNA文库中鉴定出一个编码环状指（C3HC4型锌指）蛋白的新基因。该cDNA克隆由1919个核苷酸组成，其开放阅读框编码一个485个氨基酸的蛋白质。通过逆转录（RT）-聚合酶链反应（PCR）分析，该信使RNA在各种成人人类组织中普遍表达。利用人/啮齿动物单染色体杂交细胞系和辐射杂种图谱绘制板，通过基于PCR的分析确定了该基因在染色体6p21.3区域的染色体定位。
