Akashi H, Han H J, Iizaka M, Nakajima Y, Furukawa Y, Sugano S, Imai K, Nakamura Y
Laboratory of Molecular Medicine, University of Tokyo, Japan.
J Hum Genet. 2000;45(1):43-6. doi: 10.1007/s100380050008.
Through large-scale sequencing of clones randomly selected from a library of human cDNAs, we have isolated a novel human gene termed hUQCR10. Its open reading frame encodes 63 amino acids that share 88.5% identity with the sequence of bovine ubiquinol-cytochrome C reductase 7.2-kDa protein (subunit X). A single 0.6-kb transcript was expressed in all human tissues examined, but was particularly abundant in heart and skeletal muscle, tissues that consume a large amount of oxygen. The gene product therefore may play a significant role in the cellular respiratory system. In support of this hypothesis, our immunohistochemical analysis revealed that the hUQCR10 protein is located in mitochondria. A homology search using computer programs determined the chromosomal localization of the gene at 22q12.
通过对从人类cDNA文库中随机选择的克隆进行大规模测序，我们分离出了一个名为hUQCR10的新型人类基因。其开放阅读框编码63个氨基酸，与牛泛醇 - 细胞色素C还原酶7.2 kDa蛋白（亚基X）的序列具有88.5%的同一性。在所检测的所有人类组织中均表达了单一的0.6 kb转录本，但在心脏和骨骼肌中尤为丰富，而心脏和骨骼肌是消耗大量氧气的组织。因此，该基因产物可能在细胞呼吸系统中发挥重要作用。支持这一假设的是，我们的免疫组织化学分析表明hUQCR10蛋白位于线粒体中。使用计算机程序进行的同源性搜索确定该基因定位于22q12染色体上。
