干扰素-γ对子宫内膜基质细胞分泌血管内皮生长因子的影响。
Effects of interferon-gamma on secretion of vascular endothelial growth factor by endometrial stromal cells.
作者信息
Kawano Y, Matsui N, Kamihigashi S, Narahara H, Miyakawa I
机构信息
Department of Obstetrics and Gynecology, Oita Medical University, Japan.
出版信息
Am J Reprod Immunol. 2000 Jan;43(1):47-52. doi: 10.1111/j.8755-8920.2000.430109.x.
PROBLEM
The aim of this study was to clarify the physiological effects of interferon (IFN)-gamma on secretion of vascular endothelial growth factor (VEGF) by endometrial stromal (ES) cells.
METHOD OF STUDY
ES cells were obtained from human uterine endometrium by enzymic digestion and filtration. The effects of IFN-gamma on production of VEGF by ES cells were examined by analyzing VEGF mRNA expression with Northern blotting analysis and by assaying VEGF protein.
RESULTS
IFN-gamma inhibited VEGF mRNA and protein expression by ES cells in a dose-dependent manner. In ES cells treated with IFN-gamma, VEGF production was not significant until 6 hr of incubation and was significantly affected after 6 hr of incubation, but decreased significantly after 12 to 48 hr. IFN-gamma also suppressed VEGF mRNA expression by ES cells.
CONCLUSIONS
ES cells produce VEGF, which may contribute to endometrial neovascularization and proliferation. IFN-gamma may play an important role in regulating VEGF production by ES cells.
问题
本研究的目的是阐明干扰素（IFN）-γ对子宫内膜基质（ES）细胞分泌血管内皮生长因子（VEGF）的生理作用。
研究方法
通过酶消化和过滤从人子宫内膜获取ES细胞。通过Northern印迹分析VEGF mRNA表达以及检测VEGF蛋白，来研究IFN-γ对ES细胞产生VEGF的影响。
结果
IFN-γ以剂量依赖的方式抑制ES细胞的VEGF mRNA和蛋白表达。在用IFN-γ处理的ES细胞中，孵育6小时前VEGF产生不显著，孵育6小时后受到显著影响，但在12至48小时后显著下降。IFN-γ也抑制ES细胞的VEGF mRNA表达。
结论
ES细胞产生VEGF，这可能有助于子宫内膜新生血管形成和增殖。IFN-γ可能在调节ES细胞产生VEGF中起重要作用。
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