使用亚硒酸盐F和穆勒-考夫曼四硫磺酸盐从受污水污染的河水中分离沙门氏菌。
Isolation of salmonellas from sewage-polluted river water using selenite F and Muller-Kauffmann tetrathionate.
作者信息
Harvey R W, Price T H
出版信息
J Hyg (Lond). 1976 Dec;77(3):333-9. doi: 10.1017/s0022172400055698.
Abstract
Selenite F broth and a modified Muller-Kauffmann tetrathionate broth were investigated using sewage-polluted natural water as inocula. The modification of the tetrathionate medium was necessary as commercial alternatives would not allow multiplication of small numbers of salmonellas. The wide range of molar concentrations in different tetrathionate broths was emphasized. Selenite F broth was more efficient than Muller-Kauffmann tetrathionate broth, in our hands, with the material tested. Direct inoculation of the enrichment media was used. Each of the two media examined had a bias towards selection of certain serotypes. If possible, both enrichment broths should be used to obtain maximum information.
摘要
使用受污水污染的天然水作为接种物，对亚硒酸盐F肉汤和改良的穆勒-考夫曼四硫磺酸盐肉汤进行了研究。由于市售的替代培养基无法使少量沙门氏菌繁殖，因此有必要对四硫磺酸盐培养基进行改良。强调了不同四硫磺酸盐肉汤中摩尔浓度范围很广。在我们的实验中，对于所测试的材料，亚硒酸盐F肉汤比穆勒-考夫曼四硫磺酸盐肉汤更有效。采用了直接接种富集培养基的方法。所检测的两种培养基对某些血清型的选择都有偏向性。如果可能的话，应同时使用两种富集肉汤以获取最大信息量。
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本文引用的文献
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The evaluation of culture media for the isolation of salmonellae from faeces.用于从粪便中分离沙门氏菌的培养基评估
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Effect of various enrichment broths and selective agars upon the growth of several species of Salmonella.各种增菌肉汤和选择性琼脂对几种沙门氏菌生长的影响。
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Salmonella isolation in nine European laboratories using a standardized technique.九个欧洲实验室采用标准化技术进行沙门氏菌分离。
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Elevated temperature incubation of enrichment media for the isolation of salmonellas from heavily contaminated materials.用于从严重污染材料中分离沙门氏菌的增菌培养基的高温培养。
J Hyg (Lond). 1968 Sep;66(3):377-81. doi: 10.1017/s0022172400041243.
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Incidence of river water of Escherichia coli containing R factors.含有R因子的大肠杆菌在河水中的发生率。
Nature. 1970 Dec 26;228(5278):1286-8. doi: 10.1038/2281286a0.
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The examination of samples infected with multiple salmonella serotypes.对感染多种沙门氏菌血清型的样本进行检测。
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