使用预富集技术比较亚硒酸盐F、穆勒-考夫曼四硫磺酸盐和拉帕波特培养基从受污水污染的天然水中分离沙门氏菌的效果。
Comparison of selenite F, Muller-Kauffmann tetrathionate and Rappaport's medium for the isolation of salmonellas from sewage-polluted natural water using a pre-enrichment technique.
作者信息
Harvey R W, Price T H, Xirouchaki E
出版信息
J Hyg (Lond). 1979 Dec;83(3):451-60. doi: 10.1017/s0022172400026292.
Abstract
Three enrichment broths, selenite F, Muller-Kauffmann tetrathionate and Rappaport, were examined for their efficiency in salmonella isolation. The three media, prepared from single ingredients in the laboratory, were compared with their commercial equivalents. Laboratory-prepared media were more efficient for isolating salmonellas from sewage-polluted natural water samples. A pre-enrichment stage using buffered peptone water was employed throughout the investigation. The size of inoculum from the pre-enrichment medium was relevant to successful salmonella isolation. Inocula studied were 1 ml and one loopful (3 mm diameter loop). The smaller inoculum gave better results with Rappaport, the larger with selenite and tetrathionate. Using the optimal inocula, Rappaport was the most efficient enrichment broth of the three fluid media in this study.
摘要
对三种增菌肉汤——亚硒酸盐F、穆勒-考夫曼四硫磺酸盐肉汤和拉帕波特肉汤进行了沙门氏菌分离效率检测。这三种由实验室单一成分制备的培养基与市售同类产品进行了比较。实验室制备的培养基从受污水污染的天然水样中分离沙门氏菌的效率更高。在整个研究过程中采用了用缓冲蛋白胨水进行预增菌的阶段。预增菌培养基的接种量大小与沙门氏菌的成功分离有关。所研究的接种量为1毫升和一环（直径3毫米的接种环）。较小接种量在拉帕波特肉汤中效果更好，较大接种量在亚硒酸盐肉汤和四硫磺酸盐肉汤中效果更好。使用最佳接种量时，拉帕波特肉汤是本研究中三种液体培养基中最有效的增菌肉汤。
相似文献
1
Comparison of selenite F, Muller-Kauffmann tetrathionate and Rappaport's medium for the isolation of salmonellas from sewage-polluted natural water using a pre-enrichment technique.使用预富集技术比较亚硒酸盐F、穆勒-考夫曼四硫磺酸盐和拉帕波特培养基从受污水污染的天然水中分离沙门氏菌的效果。
J Hyg (Lond). 1979 Dec;83(3):451-60. doi: 10.1017/s0022172400026292.
2
Comparison of selenite F, Muller-Kauffmann tetrathionate and Rappaport's medium for salmonella isolation from chicken giblets after pre-enrichment in buffered peptone water.在缓冲蛋白胨水中预富集后，比较亚硒酸盐F、穆勒-考夫曼四硫代硫酸盐和拉帕波特培养基用于从鸡内脏中分离沙门氏菌的效果。
J Hyg (Lond). 1981 Oct;87(2):219-24. doi: 10.1017/s0022172400069436.
3
Isolation of salmonellas from sewage-polluted river water using selenite F and Muller-Kauffmann tetrathionate.使用亚硒酸盐F和穆勒-考夫曼四硫磺酸盐从受污水污染的河水中分离沙门氏菌。
J Hyg (Lond). 1976 Dec;77(3):333-9. doi: 10.1017/s0022172400055698.
4
A comparison of two modifications of Rappaport's enrichment medium (R25 and RV) for the isolation of salmonellas from sewage polluted natural water.比较用于从受污水污染的天然水中分离沙门氏菌的两种改良拉帕波特富集培养基（R25和RV）。
J Hyg (Lond). 1983 Dec;91(3):451-8. doi: 10.1017/s0022172400060496.
5
A comparison of tetrathionate broth and Rappaport's medium as enrichment media for Salmonella.作为沙门氏菌增菌培养基的连四硫酸盐肉汤与拉帕波特培养基的比较。
Nord Vet Med. 1977 Mar;29(3):141-3.
6
Comparison of Muller-Kauffmann tetrathionate broth with Rappaport-Vassiliadis (RV) medium for the isolation of salmonellas from sewage sludge.比较穆勒 - 考夫曼四硫磺酸盐肉汤培养基与拉帕波特 - 瓦西里迪斯（RV）培养基用于从污水污泥中分离沙门氏菌的效果。
J Appl Bacteriol. 1986 Feb;60(2):161-7. doi: 10.1111/j.1365-2672.1986.tb03374.x.
7
Salmonella isolation with Rappaport's medium after pre-enrichment in buffered peptone water using a series of inoculum ratios.在缓冲蛋白胨水中进行预富集后，使用一系列接种比例，用拉帕波特培养基分离沙门氏菌。
J Hyg (Lond). 1980 Aug;85(1):125-8. doi: 10.1017/s0022172400027133.
8
A comparison of isolation procedures for salmonellas from polluted water using two forms of Rappaport's medium.使用两种形式的拉帕波特培养基对污染水中沙门氏菌分离程序的比较。
J Appl Bacteriol. 1984 Apr;56(2):305-9. doi: 10.1111/j.1365-2672.1984.tb01351.x.
9
A comparisoin of the R25 modification of Rappaport s enrichment medium with strontium chloride B for salmonella isolation from sewage polluted natural water.用于从受污水污染的天然水中分离沙门氏菌的拉帕波特富集培养基R25改良版与氯化锶B的比较。
J Hyg (Lond). 1982 Aug;89(1):111-7. doi: 10.1017/s0022172400070595.
10
Observations on pre-enrichment for isolating salmonellas from sewage polluted natural water using Muller-Kauffmann tetrathionate broth prepared with fresh and desiccated ox bile.
J Appl Bacteriol. 1977 Aug;43(1):145-8. doi: 10.1111/j.1365-2672.1977.tb00733.x.
引用本文的文献
1
Growth kinetics of Salmonella enterica in Hajna tetrathionate broth, Rappaport broth and modified semisolid Rappaport agar.肠炎沙门氏菌在哈伊纳四硫磺酸盐肉汤、雷帕波特肉汤和改良半固体雷帕波特琼脂中的生长动力学。
J Vet Med Sci. 2016 Mar;78(3):435-8. doi: 10.1292/jvms.15-0342. Epub 2015 Oct 24.
2
Improved isolation of Salmonellae from naturally contaminated meat products by using Rappaport-Vassiliadis enrichment broth.通过使用Rappaport-Vassiliadis增菌肉汤改进从天然污染的肉制品中分离沙门氏菌的方法。
Appl Environ Microbiol. 1981 Oct;42(4):615-8. doi: 10.1128/aem.42.4.615-618.1981.
3
Isolation of Salmonella with the use of 100 ml of the R10 modification of Rappaport's enrichment medium.使用100毫升拉帕波特富集培养基的R10改良培养基分离沙门氏菌。
J Hyg (Lond). 1981 Aug;87(1):35-41. doi: 10.1017/s0022172400069217.
4
A comparisoin of the R25 modification of Rappaport s enrichment medium with strontium chloride B for salmonella isolation from sewage polluted natural water.用于从受污水污染的天然水中分离沙门氏菌的拉帕波特富集培养基R25改良版与氯化锶B的比较。
J Hyg (Lond). 1982 Aug;89(1):111-7. doi: 10.1017/s0022172400070595.
5
Comparison of selenite F, Muller-Kauffmann tetrathionate and Rappaport's medium for salmonella isolation from chicken giblets after pre-enrichment in buffered peptone water.在缓冲蛋白胨水中预富集后，比较亚硒酸盐F、穆勒-考夫曼四硫代硫酸盐和拉帕波特培养基用于从鸡内脏中分离沙门氏菌的效果。
J Hyg (Lond). 1981 Oct;87(2):219-24. doi: 10.1017/s0022172400069436.
6
Salmonella isolation with Rappaport's medium after pre-enrichment in buffered peptone water using a series of inoculum ratios.在缓冲蛋白胨水中进行预富集后，使用一系列接种比例，用拉帕波特培养基分离沙门氏菌。
J Hyg (Lond). 1980 Aug;85(1):125-8. doi: 10.1017/s0022172400027133.
7
A comparison of the original Rappaport medium (R medium) and the Rappaport-Vassiliadis medium (RV medium) in the isolation of salmonellae from meat products.用于从肉制品中分离沙门氏菌的原始拉帕波特培养基（R培养基）与拉帕波特-瓦西里亚迪斯培养基（RV培养基）的比较。
J Hyg (Lond). 1984 Aug;93(1):51-8. doi: 10.1017/s0022172400060927.
8
The effect of the use of different selective media on the ability to recover salmonellae from seagull faeces.使用不同选择性培养基对从海鸥粪便中分离沙门氏菌能力的影响。
J Hyg (Lond). 1984 Aug;93(1):35-42. doi: 10.1017/s0022172400060903.
9
A comparison of two modifications of Rappaport's enrichment medium (R25 and RV) for the isolation of salmonellas from sewage polluted natural water.比较用于从受污水污染的天然水中分离沙门氏菌的两种改良拉帕波特富集培养基（R25和RV）。
J Hyg (Lond). 1983 Dec;91(3):451-8. doi: 10.1017/s0022172400060496.
10
Effect of stool storage at room temperature on salmonella isolation from faeces.粪便室温保存对粪便中沙门氏菌分离的影响。
J Hyg (Lond). 1983 Oct;91(2):267-71. doi: 10.1017/s0022172400060277.
本文引用的文献
1
The numbers of pathogenic bacilli in faeces in intestinal diseases.肠道疾病患者粪便中致病杆菌的数量。
J Hyg (Lond). 1955 Jun;53(2):217-24. doi: 10.1017/s0022172400000681.
2
AN IMPROVED METHOD OF ISOLATING SALMONELLAE FROM CONTAMINATED DESICCATED COCONUT.一种从受污染的干椰子中分离沙门氏菌的改良方法。
J Clin Pathol. 1964 Jan;17(1):75-8. doi: 10.1136/jcp.17.1.75.
3
A trial of Rappaport's medium.对拉帕波特培养基的一项试验。
J Clin Pathol. 1958 Sep;11(5):426-7. doi: 10.1136/jcp.11.5.426.
4
A new enrichment medium for certain Salmonellae.一种用于某些沙门氏菌的新型富集培养基。
J Clin Pathol. 1956 Aug;9(3):261-6. doi: 10.1136/jcp.9.3.261.
5
Choice of a selective medium for the routine isolation of members of the Salmonella group.用于常规分离沙门氏菌群成员的选择性培养基的选择。
Mon Bull Minist Health Public Health Lab Serv. 1956 Jun;15:118-24.
6
Optimum temperature of incubation for isolation of Salmonellae.用于分离沙门氏菌的最佳培养温度。
Mon Bull Minist Health Public Health Lab Serv. 1953 Jul;12:149-50.
7
A comparative trial of Rappaport enrichment medium for the isolation of Salmonellae from faeces.用于从粪便中分离沙门氏菌的拉帕波特增菌培养基的比较试验。
J Clin Pathol. 1967 May;20(3):290-3. doi: 10.1136/jcp.20.3.290.
8
Salmonella isolation in nine European laboratories using a standardized technique.九个欧洲实验室采用标准化技术进行沙门氏菌分离。
Bull World Health Organ. 1969;41(2):297-306.
9
An evaluation of Rappaport's magnesium chloride/malachite green medium in the routine examination of faeces.拉帕波特氯化镁/孔雀绿培养基在粪便常规检查中的评估
J Hyg (Lond). 1965 Dec;63(4):491-5. doi: 10.1017/s002217240004537x.
10
Salmonella isolations in abattoirs in Greece.希腊屠宰场中的沙门氏菌分离情况。
J Hyg (Lond). 1970 Dec;68(4):601-9. doi: 10.1017/s0022172400042534.
Zotero 插件