使用预富集技术比较亚硒酸盐F、穆勒-考夫曼四硫磺酸盐和拉帕波特培养基从受污水污染的天然水中分离沙门氏菌的效果。
Comparison of selenite F, Muller-Kauffmann tetrathionate and Rappaport's medium for the isolation of salmonellas from sewage-polluted natural water using a pre-enrichment technique.
作者信息
Harvey R W, Price T H, Xirouchaki E
出版信息
J Hyg (Lond). 1979 Dec;83(3):451-60. doi: 10.1017/s0022172400026292.
Abstract
Three enrichment broths, selenite F, Muller-Kauffmann tetrathionate and Rappaport, were examined for their efficiency in salmonella isolation. The three media, prepared from single ingredients in the laboratory, were compared with their commercial equivalents. Laboratory-prepared media were more efficient for isolating salmonellas from sewage-polluted natural water samples. A pre-enrichment stage using buffered peptone water was employed throughout the investigation. The size of inoculum from the pre-enrichment medium was relevant to successful salmonella isolation. Inocula studied were 1 ml and one loopful (3 mm diameter loop). The smaller inoculum gave better results with Rappaport, the larger with selenite and tetrathionate. Using the optimal inocula, Rappaport was the most efficient enrichment broth of the three fluid media in this study.
摘要
对三种增菌肉汤——亚硒酸盐F、穆勒-考夫曼四硫磺酸盐肉汤和拉帕波特肉汤进行了沙门氏菌分离效率检测。这三种由实验室单一成分制备的培养基与市售同类产品进行了比较。实验室制备的培养基从受污水污染的天然水样中分离沙门氏菌的效率更高。在整个研究过程中采用了用缓冲蛋白胨水进行预增菌的阶段。预增菌培养基的接种量大小与沙门氏菌的成功分离有关。所研究的接种量为1毫升和一环（直径3毫米的接种环）。较小接种量在拉帕波特肉汤中效果更好，较大接种量在亚硒酸盐肉汤和四硫磺酸盐肉汤中效果更好。使用最佳接种量时，拉帕波特肉汤是本研究中三种液体培养基中最有效的增菌肉汤。
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